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In recent years, the application of nanotechnology to biomedicine has been exponentially increasing. The physical and chemical properties, quality and safety of nanomaterials designed for biomedical application need to be accurately evaluated by means of reliable and robust techniques. Among the methods used, microscopy techniques play a primary role. This paper presents a brief overview of the contribution of different microscopy techniques to the study of the structural and functional aspects of nanoconstructs and their relationships with the biological milieu, demonstrating the great impact that microscopy sciences have in nanomedical research and applications.
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