Application of confocal laser scanning microscopy in the taxonomy of free-living marine nematodes


https://doi.org/10.4081/microscopie.2016.6309
Vol. 26 No. 2 (2016)
Submitted: 27 September 2016
Accepted: 27 September 2016
Published: 27 September 2016
taxonomy, free-living marine nematodes, confocal laser scanning microscopy, autofluorescence
Abstract Views: 873
PDF: 1077
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Authors

  • F. Semprucci
    federica.semprucci@uniurb.it
    Department of Biomolecular Sciences (DiSB), University of Urbino, Italy.
  • S. Burattini Department of Biomolecular Sciences (DiSB), University of Urbino, Italy.
  • H. Kim Department of Environmental Science, College of Natural Sciences, Hanyang University, Seoul 04763, Korea, Democratic People's Republic of.
  • J.H. Hong Biodiversity Research Institute, Marine Act co., Seoul 04790, Korea, Democratic People's Republic of.
  • W. Lee Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, Korea, Democratic People's Republic of.
  • L. Guidi Department of Biomolecular Sciences (DiSB), University of Urbino, Italy.
  • E. Falcieri Department of Biomolecular Sciences (DiSB), University of Urbino, Italy.
  • M. Balsamo Department of Biomolecular Sciences (DiSB), University of Urbino, Italy.
Confocal laser scanning microscopy (CLSM) can provide high-resolution images of thick nematode specimens and highly detailed three-dimensional reconstructions. Several permanent slides of free-living marine nematode species including a species collected in the 1951 were analyzed with the aid of CLSM. The specimens were excited using an argon laser at 488 nm under the conditions used for Fluorescein Isothiocyanate (FITC) and 543 nm for Tetramethyl Rhodamine Isothiocyanate (TRITC). New details on the morphology of various diagnostic features (the cephalic region and male reproductive system) have been captured for several species allowing a re-description for some of them. Spicules and gubernaculum, followed by cuticularised parts of the buccal cavity and precloacal supplements were the most fluorescent parts of the nematode body. The morphological approach here adopted highlights new chances for the study of Museum type material for which CLSM may be decisive in capturing additional, important taxonomical details. Material collected in the 1951 and 1973 still resulted fluorescent making possible the detection of crucial taxonomical data.

Semprucci, F., Burattini, S., Kim, H., Hong, J., Lee, W., Guidi, L., Falcieri, E., & Balsamo, M. (2016). Application of confocal laser scanning microscopy in the taxonomy of free-living marine nematodes. Microscopie, 26(2), 48–57. https://doi.org/10.4081/microscopie.2016.6309

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