ANTIOXIDANT EFFECT OF RESVERATROL IN HUMAN SPERMATOZOA AND IN RAT GERMINAL CELLS
AbstractObjective: To assess the antioxidant activity of Resveratrol (3,5,4’-trihydroxystilbene, RES) after induction of lipid peroxidation LPO) in human spermatozoa and in immature rat germinal cells. Materials and Methods: Ejaculated human spermatozoa, selected by swim up, have been incubated with tert-Butylhydroperoxide and tert-Butylhydroperoxide-RES. The localization of LPO has been performed using the probe C11-BODIPY581/591. The same assays were carried out on pachytene spermatocytes and round spermatids obtained from three Wistar rats 35 days of age. The two cellular fractions were achieved after enzymatic digestion with collagenase and subsequent fractionation on bovine serum albumin 0.5-3% gradient (STAPUT). The ultrastructure of all samples was assessed by transmission electron microscopy (TEM). Results: The midpiece of sperm tail and the whole plasma membrane of germ cells were the target of LPO. TEM analysis of sperm, quantitatively elaborated by a mathematical formula, showed a significantly lower percentage of necrosis in the samples treated with RES (P<0.01); as regards rat germinal cells, necrosis features (cytoplasmic vacuoles, disrupted chromatin and broken plasma membrane) were mainly evident in the meiotic fraction without RES. Conclusions: RES, found in the skins of grape, reduces the damage induced by oxidative stress in human sperm and rat testicular germ cells; in particular spermatids appeared to be less sensitive to oxidative damage compared with spermatocytes.
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Copyright (c) 2012 MG. Federico, G. Collodel, T. Renieri, NA. Pascarelli, M. Geminiani, S. Martini, C. Bonechi, C. Rossi, E. Moretti
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