https://doi.org/10.4081/jear.2021.9400
Bio efficacy of Cinnamaldehyde from Cinnamomum verum essential oil against Culex quinquefasciatus (Diptera: Culicidae)
Submitted: 13 October 2020
Accepted: 27 January 2021
Published: 10 May 2021
Accepted: 27 January 2021
Abstract Views: 3455
PDF: 936
HTML: 213
HTML: 213
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
This research aims to study the efficacy of Cinnamomum verum (Cv) extracts for ovicidal, larvicidal, and repellent activities against Culex quinquefasciatus mosquito vectors. The active components of C. verum or cinnamon oil by Gas Chromatography (GC) analysis showed the highest cinnamaldehyde at 83.53%. For ovicidal assay, C. verum essential oil at concentrations 12.5, 25 and 50 ppm at 72 h had 100% egg hatch inhibition and had a significant difference when compared to the control group (p<0.05). Larvicidal activity showed that concentrations of 25 and 50 ppm were highly effective in killing 100% mosquito larvae. Morphological changes in egg raft showed a faded color and eggs that seemed to have split from their raft while the larvae changed to a pale white wrinkled body with a destroyed inner tube of the body and were motionless. A Scanning electron microscope study showed that the eggshell and micropyle were wrinkled with the chitin peeled out. After treatment with cinnamon oil, larvae appeared to have a wrinkled body, the thorax and abdominal cuticle were also destroyed with high density of oil particles observed on mouth brushes and obstructing the spiracle. The repellent assay showed that cinnamon oil could repel both male and female mosquitos for up to 180 min. From the results, it was concluded that cinnamon oil had highly effective repellency against Culex quinquefasciatus adults and insecticidal activity on eggs and larvae stages evidenced by LC50 at 6.59±0.54, 9.07±0.67 and 36.91±7.56 ppm and its morphological changes indicated how the mosquito could not survive after cinnamon oil treatment hence this may be a useful alternative method that is green friendly for controlling mosquitoes in endemic areas.
ABDELGALEIL, S.A., MOHAMED, M.I., BADAWY, M.E., ELARAMI, S.A., 2009- Fumigant and contact toxicities of monoterpenes to Sitophilus oryzae (L.) and Tribolium castaneum (Herbst) and their inhibitory effects on acetylcholinesterase activity.- J. Chem. Ecol. 35: 518-525.
ABTEW, A., SUBREMANIAN, S., CHESETO, X., KREITER, S., GARZIA, G.T., MARTIN, T., 2015-Repellency of Plant Extracts against the Legume Flower Thrips Megalurothrips sjostedti (Thysanoptera: Thripidae).-Insects. 6: 608-625.
AGUITAR, R.W., DOS SANTOS, S.F., DA SILVA MORGADO, F., ASCENCIO, S.D., DE MEDONCA LOPES, M., VIANA, K.F., DIDONET, J., RIBEIRO, B.M., 2015-Insecticidal and repellent activity of Siparuna guianensis Aubl. (Negramina) against Aedes aegypti and Culex quinquefasciatus. -PLoS One. 10: e0116765.
AMER, A., MEHLHORN, H.,- 2006. Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae).-Parasitol. Res. 99: 466-472.
BATIHA, G.E., BESHBISHY, A.M., GUSWANTO, A., NUGRAHA, A., MUNKHJARGAL, T., M, M.A.-D., MOSQUEDA, J., IGARASHI, I., 2020.- Phytochemical Characterization and Chemotherapeutic Potential of Cinnamomum verum Extracts on the Multiplication of Protozoan Parasites In Vitro and In Vivo. Molecules.- 25.
BOTAS, G.D.S., CRUZ, R.A.S., DE ALMEIDA, F.B., DUARTE, J.L., ARAUJO, R.S., SOUTO, R.N.P., FERREIRA, R., CARVALHO, J.C.T., SANTOS, M.G., ROCHA, L., PEREIRA, V.L.P., FERNANDES, C.P., 2017.-Baccharis reticularia DC. and Limonene Nanoemulsions: Promising Larvicidal Agents for Aedes aegypti (Diptera: Culicidae) Control.- Molecules. 22.
CHAITHONG, U., CHOOCHOTE, W., KAMSUK, K., JITPAKDI, A., TIPPAWANGKOSOL, P., CHAIYASIT, D., CHAMPAKAEW, D., TUETUN, B., PITASAWAT, B., 2006. -Larvicidal effect of pepper plants on Aedes aegypti (L.) (Diptera: Culicidae).- J. Vector. Ecol. 31: 138-144.
CHOOCHOTE, W., TUETUN, B., KANJANAPOTHI, D., RATTANACHANPICHAI, E., CHAITHONG, U., CHAIWONG, P., JITPAKDI, A., TIPPAWANGKOSOL, P., RIYONG, D., PITASAWAT, B., 2004. -Potential of crude seed extract of celery, Apium graveolens L., against the mosquito Aedes aegypti (L.) (Diptera: Culicidae)-. J. Vector. Ecol. 29: 340-346.
CORBEL, V., STANKIEWICZ, M., PENNETIER, C., FOURNIER, D., STOJAN, J., GIRARD, E., DIMITROV, M., MOLGO, J., HOUGARD, J.M., LAPIED, B., 2009.- Evidence for inhibition of cholinesterases in insect and mammalian nervous systems by the insect repellent deet.- BMC. Biol. 7: 47.
DEGENNARO, M., MCBRIDE, C.S., SEEHOLZER, L., NAKAGAWA, T., DENNIS, E.J., GOLDMAN, C., JASINSKIENE, N., JAMES, A.A., VOSSHALL, L.B., 2013.- Orco mutant mosquitoes lose strong preference for humans and are not repelled by volatile DEET-. Nature. 498: 487-491.
DELETRE, E., CHANDRE, F., WILLIAMS, L., DUMENIL, C., MENUT, C., MARTIN, T., 2015.- Electrophysiological and behavioral characterization of bioactive compounds of the Thymus vulgaris, Cymbopogon winterianus, Cuminum cyminum and Cinnamomum zeylanicum essential oils against Anopheles gambiae and prospects for their use as bednet treatments.- Parasit. Vectors. 8: 316.
EMILIE, D., MALLENT, M., MENUT, C., CHANDRE, F., MARTIN, T., 2015.- Behavioral Response of Bemisia tabaci (Hemiptera: Aleyrodidae) to 20 Plant Extracts.- J. Econ. Entomol. 108: 1890-1901.
FOUAD, H., HONGJIE, L., HOSNI, D., WEI, J., ABBAS, G., GAAL, H., JIANCHU, M., 2018.- Controlling Aedes albopictus and Culex pipiens pallens using silver nanoparticles synthesized from aqueous extract of Cassia fistula fruit pulp and its mode of action. -Artif. Cells. Nanomed. Biotechnol. 46: 558-567.
FUKUTO, T.R., 1990.- Mechanism of action of organophosphorus and carbamate insecticides. Environ. Health. Perspect. 87: 245-254.
GOVINDARAJAN, M., 2011. -Mosquito larvicidal and ovicidal activity of Cardiospermum halicacabum Linn. (family: Sapindaceae) leaf extract against Culex quinquefasciatus (Say.) and Aedes aegypti (Linn.) (Diptera: Culicidae).- Eur. Rev. Med. Pharmacol. Sci. 15: 787-794.
HAMIDPOUR, R., HAMIDPOUR, M., HAMIDPOUR, S., Shahlari, M., 2015.- Cinnamon from the selection of traditional applications to its novel effects on the inhibition of angiogenesis in cancer cells and prevention of Alzheimer's disease, and a series of functions such as antioxidant, anticholesterol, antidiabetes, antibacterial, antifungal, nematicidal, acaracidal, and repellent activities.- J. Tradit. Complement. Med. 5: 66-70.
KAIN, P., BOYLE, S.M., THARADRA, S.K., GUDA, T., PHAM, C., DAHANUKAR, A., Ray, A., 2013. -Odour receptors and neurons for DEET and new insect repellents.- (Retracted article. See. vol. 536, 2016). Nature. 502: 507-512.
KALAIVANI, K., SENTHIL-NATHAN, S., MURUGESAN, A.G., 2012. -Biological activity of selected Lamiaceae and Zingiberaceae plant essential oils against the dengue vector Aedes aegypti L. (Diptera: Culicidae)-. Parasitol. Res. 110: 1261-1268.
KALLEL, I., HADRICH, B., GARGOURI, B., CHAABANE, A., LASSOUED, S., GDOURA, R., BAYOUDH, A., BEN MESSAOUD, E., 2019. -Optimization of Cinnamon (Cinnamomum zeylanicum Blume) Essential Oil Extraction: Evaluation of Antioxidant and Antiproliferative Effects. -Evid. Based. Complement. Alternat. Med. 6498347. https://doi.org/10.1155/2019/6498347.
DOI: https://doi.org/10.1155/2019/6498347
KHATER, H.F., GEDEN, C.J., 2018. -Potential of essential oils to prevent fly strike and their effects on the longevity of adult Lucilia sericata.- J. Vector. Ecol. 43: 261-270.
KRINGER, R., 2010. Handbook of pesticide toxicology. Academic Press.
LIANG, Y., LI, Y., SUN, A., LIU, X., 2019.- Chemical compound identification and antibacterial activity evaluation of cinnamon extracts obtained by subcritical n-butane and ethanol extraction. -Food. Sci. Nutr. 7: 2186-2193.
MADRESEH-GHAHFAROKHI, S., PIRALI, Y., DEHGHANI-SAMANI, A., 2018.- The insecticidal and repellent activity of ginger (Zingiber officinale) and eucalyptus (Eucalyptus globulus) essential oils against Culex theileri Theobald, 1903 (Diptera: Culicidae.- Ann. Parasitol. 64: 351-360.
MCIVER, S., 1978. Structure of sensilla trichodea of female Aedes aegypti with comments on innervation of antennal sensilla.- J. Insect. Physiol. 24: 383-390.
NABAVI, S.F., DI LORENZO, A., IZADI, M., SOBARZO-SANCHEZ, E., DAGLIA, M., NABAVI, S.M., 2015. Antibacterial Effects of Cinnamon: From Farm to Food, Cosmetic and Pharmaceutical Industries.- Nutrients. 7: 7729-7748.
Phasomkusolsil, S., Soonwera, M., 2011. Efficacy of herbal essential oils as insecticide against Aedes aegypti (Linn.), Culex quinquefasciatus (Say) and Anopheles dirus (Peyton and Harrison). Southeast Asian J Trop Med Public Health 42, 1083-1092.
RAMKUMAR, G., KARTHI, S., SHIVAKUMAR, M.S., KWEKA, E.J., 2019. Culex quinquefasciatus Egg Membrane Alteration and Ovicidal Activity of Cipadessa baccifera (Roth) Plant Extracts Compared to Synthetic Insect Growth Regulators-. Res Rep. Trop. Med. 10: 145-151.
RANASINGHE, P., GALAPPATHTHY, P., CONSTANTINE, G.R., JAYAWARDENA, R., WEERATUNGA, H.D., PREMAKUMARA, S., KATULANDA, P., 2017.- Cinnamomum zeylanicum (Ceylon cinnamon) as a potential pharmaceutical agent for type-2 diabetes mellitus: study protocol for a randomized controlled trial.- Trials. 18:446.
REEGAN, A.D., GANDHI, M.R., PAULRAJ, M.G., Ignacimuthu, S., 2015. -Ovicidal and Oviposition Deterrent Activities of Medicinal Plant Extracts Against Aedes aegypti L. and Culex quinquefasciatus Say Mosquitoes (Diptera: Culicidae).- Osong. Public. Health. Res. Perspect. 6: 64-69.
SAEMA, D.K., MOHAPATRE, P.K., BHATTACHARYYA, D.R., CHELLAPPAN, S., KARUPPUSAMY, B., BARMAN, K., SENTHIL KUMAR, N., DASH, A.P., PRAKASH, A., BALABASKARAN NINA, P., 2019.- Malaria in North-East India: Importance and Implications in the Era of Elimination. Microorganisms. 7.
SEENIVASAGAN, T., SHARMA, K.R., SHRIVASTAVA, A., PARASHAR, B.D., PANT, S.C., PRAKASH, S., 2009. Surface morphology and morphometric analysis of sensilla of Asian tiger mosquito, Aedes albopictus (Skuse): an SEM investigation. -J. Vector. Borne.Dis. 46: 125-135.
SENANAYAKE, U.M., LEE, T.H., WILLS, R.B.H., 1987.- Volatile constituents of cinnamon (Cinnamomum zeylanicum) oils.- J. Agric. Food. Chem. 26: 822-824.
SHU Z, X. L., JIE L, VAN DER, H, W., 2008. CINNAMOMUM Flora. China 7: 166-187.
SINGH, G., MAURYA, S., DELAMPASONA, M.P., CATALAN, C.A., 2007. -A comparison of chemical, antioxidant and antimicrobial studies of cinnamon leaf and bark volatile oils, oleoresins and their constituents.- Food. Chem. Toxicol. 45: 1650-1661.
SOONWERA, M., SITTICHOK, S., 2020.- Adulticidal activities of Cymbopogon citratus (Stapf.) and Eucalyptus globulus (Labill.) essential oils and of their synergistic combinations against Aedes aegypti (L.), Aedes albopictus (Skuse), and Musca domestica (L.).- Environ. Sci. Pollut. Res. Int. 27: 20201-20214.
SRITABUTRA, D., SOOWERA, M., WALTANACHANOBON, S., POUNGJAI, S., 2011.- Evaluation of herbal essential oil as repellents against Aedes aegypti (L.) and Anopheles dirus Peyton & Harrion.- Asian. Pac. J. Trop. Biomed. 1: S124–S128.
SUMAN, D.S., WANG, Y., BILGRAMI, A.L., GAUGLER, R., 2013.- Ovicidal activity of three insect growth regulators against Aedes and Culex mosquitoes. -Acta. Trop. 128: 103-109.
SUTININGSIH, D., NURJAZULI, N., NUGROHO, D., SATOTO, T.B.T., 2019.- Larvicidal Activity of Brusatol Isolated from Brucea javanica (L) Merr on Culex quinquefasciatus.- Iran. J. Public. Health. 48: 688-696.
THOMAS, A., MAZIGO, H.D., MANJURANO, A., MORONA, D., KWEKA, E.J., 2017.- Evaluation of active ingredients and larvicidal activity of clove and cinnamon essential oils against Anopheles gambiae (sensu lato).- Parasit. Vectors. 10: 411.
VENI, T., PUSHPANATHAN, T., MOHANRAJ, J., 2017.-Larvicidal and ovicidal activity of Terminalia chebula Retz. (Family: Combretaceae) medicinal plant extracts against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus.- J. Parasit. Dis. 41: 693-702.
WHO, 1982. Lymphatic Filariasis. Fact Sheet N°102.
WHO, 2014. A global brief on vector-borne diseases. [accessed April 12, 2020]
WIJESEKERA, R.O., 1978. -Historical overview of the cinnamon industry.- CRC. Crit. Rev Food. Sci. Nutr. 10: 1-30.
ZHANG, Q., WU, X., LIU, Z., 2016.-Primary Screening of Plant Essential Oils as Insecticides, Fumigants, and Repellents Against the Health Pest Paederus fuscipes (Coleoptera: Staphylinidae)-. J. Econ. Entomol. 109: 2388-2396.
Supporting Agencies
National research and innovation (Non-flagship) 2563How to Cite
Nakasen, K. ., Wongsrila, A. ., Prathumtet, J. ., Sriraj, P. ., Boonmars, T. ., Promsrisuk, T. ., Laikaew, N. ., & Aukkanimart, R. (2021). Bio efficacy of Cinnamaldehyde from <em>Cinnamomum verum</em> essential oil against <em>Culex quinquefasciatus</em> (Diptera: Culicidae). Journal of Entomological and Acarological Research, 53(1). https://doi.org/10.4081/jear.2021.9400
PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.