Synergistic effect of certain insecticides combined with Bacillus thuringiensis on mosquito larvae

  • C.P. Narkhede School of Life Sciences, North Maharashtra University, Maharashtra, India.
  • C.D. Patil School of Life Sciences, North Maharashtra University, Maharashtra, India.
  • R.K. Suryawanshi School of Life Sciences, North Maharashtra University, Maharashtra, India.
  • S.H. Koli School of Life Sciences, North Maharashtra University, Maharashtra, India.
  • B.V. Mohite School of Life Sciences, North Maharashtra University, Maharashtra, India.
  • S.V. Patil | satish.patil7@gmail.com School of Life Sciences, and North Maharashtra Microbial Culture Collection Centre, North Maharashtra University, Maharashtra, India.

Abstract

For effective vector control it is essential to formulate new preparations having multiple action against the vector pest. Developing combined formulation of biopesticide and chemical pesticide is one of the novel concept to fight against the vectors with new weapons; however, compatibility of biopesticide i.e. Bacillus thuringiensis (Bt) and chemical pesticide is a real hurdle. In this investigation, local isolate Bacillus thuringiensis SV2 (BtSV2) was tested for its compatibility with various available mosquito larvicides. Temephos was most compatible with BtSV2 than with other tested pesticides. These two compatible agents were tested for larvicidal potential. Our study revealed that the synergistic effect of both agents reduces LC50 value by 30.68 and 22.36% against the Ae. aegypti and An. stephensi, respectively. The larvicidal potential increased when compared to individual pesticides. It was also observed a biochemical change in larvae after the TBT (Temephos + Bacillus thuringiensis) combination treatment; it involves decreased level of alpha esterase, acetylcholine esterase and protein while level of beta esterase and acid phosphatase was unchanged and alkaline phosphatase activity was increased. Increased potential of combined formulation may be due to altered physiological condition.

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Published
2017-04-21
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Keywords:
Combination, Bacillus thuringiensis (Bt), Temephos, Esterase, Protease, Larvicidal
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How to Cite
Narkhede, C., Patil, C., Suryawanshi, R., Koli, S., Mohite, B., & Patil, S. (2017). Synergistic effect of certain insecticides combined with Bacillus thuringiensis on mosquito larvae. Journal of Entomological and Acarological Research, 49(1). https://doi.org/10.4081/jear.2017.6265