Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale 2020-07-06T21:09:39+00:00 Francesca Savio Open Journal Systems <p>The <strong>Journal of Biological Research – Bollettino della <a href="" target="_blank" rel="noopener">Società Italiana di Biologia Sperimentale</a></strong> is one of the oldest journals in Biology. Founded in 1925 the journal is available in <a href="[jour]" target="_blank" rel="noopener">Medline</a> until 2001 and <a href=";origin=resultslist" target="_blank" rel="noopener">Scopus</a> since 2014. The <strong>Journal of Biological Research – Bollettino della&nbsp;<a href="" target="_blank" rel="noopener">Società Italiana di Biologia Sperimentale</a></strong>&nbsp;is an online-only peer-reviewed journal which welcomes papers dealing with any aspect of experimental biology. Papers concerning clinical topics can be accepted only if they include experimental laboratory data. Original communications, review articles or short communications may be submitted.</p> Tetracycline Resistant Genes as Bioindicators of Water Pollution 2020-07-06T21:05:11+00:00 Manal F. Abdelall Safa S. Hafez Maryam El. Fayad Hanan A. Nour El-Din, Soad A. Abdallah <p>The current study aimed to investigate the prevalence of tetracycline resistant bacteria isolated from different water samples and the genes responsible for this resistance. Two hundred fifty isolates were isolated from different water samples from two different locations. Isolates were obtained from El-Zamalek site was (n =110) and from Rod El-Farag site was (n = 140). A hundred isolates out of 250 bacterial isolates (40%) were resistant to tetracycline at a concentration of 16 μg/ml. Only 31 (31%) were selected due to their resistance to (32 μg/ml) tetracycline for identification. All selected isolates were identified according to biochemical and the 16S sequence techniques. The 16S rDNA gene sequences of the bacterial isolates which were reported in this study were submitted to the NCBI database. Of the 31 isolates were analyzed by Polymerase Chain Reaction (PCR), results showed that 41.9 % (13/31) harbored tet A gene, 74.2% (23/31) carried tet D gene, while 12.9 % (4/31) carried tet M gene. Whereas tet B, tet C and tet O were not detected. Twenty-one isolates (67.7%) harbored a single tet gene, five isolates (16.1%) harbored two different tet genes while three isolates (9.7 %) harbored three different tet genes. Moreover, two isolates were free from any tested tet genes.</p> 2020-06-29T15:48:06+00:00 ##submission.copyrightStatement## Hairy root culture of white mulberry (Morus alba L.) for a source of tyrosinase inhibitors 2020-07-06T21:05:09+00:00 Hanh Thi My Nguyen Tan Quoc Tran Anh Lan Bui Phuong Ngo Diem Quach <p>White mulberry (<em>Morus alba</em> L.) root was reported to contain potential tyrosinase inhibitors such as norartocarpetin, streppogenin, oxyresveratrol (5, 7, 2′, 4′-tetrahydroxy-flavavone) and 2-arylbenzofuran. This study aims at investigating <em>Morus alba</em> L. hairy root culture to create a source of tyrosinase inhibitors for skin-whitening application. <em>Morus alba</em> L. leaf (including mesophyll and petiole) and stem explants were randomly wounded and inoculated with<em> Agrobacterium rhizogenes</em> ATCC 15834 in 10-40 minutes, and coculturing for 4 days. After inoculation, <em>Agrobacterium rhizogenes</em> were removed by using MS medium adding 200mg/L cefotaxime as antibiotics. Then, fresh inducted hairy roots were grown in nonphytohormone liquid medium (MS, White, Gamborg B5 or WPM) on a shaker (80rpm) at 25 ±1°C. Inducted hairy roots were tested for the present of <em>rolB</em> by PCR analysis and its effect on tyrosinase activity using a 96-well plate reader. The suitable explants for hairy root induction are leaves, especially petioles in 10 minutes (root induction frequency: 83%). After induction, hairy roots on MS was grown more efficiently (increased 3.933 times in FW) than on WPM, B5 and White medium. Inducted hairy roots were successfully transgenes by the present of <em>rolB</em> in their genome and had the same anti-tyrosinase activity as the natural roots without significance differences (IC50: 3.860 and 3.597 μg/mL, respectively). Cultured <em>Morus alba</em> L. hairy roots are potential source of tyrosinase inhibitors for future skin-whitening ingredient.</p> 2020-07-01T10:14:57+00:00 ##submission.copyrightStatement## Bio-organic and inorganic fertilizers modify leaf nutrients, essential oil properties, and antioxidant capacity in medic savory (Satureja macrantha L.) 2020-07-06T21:05:07+00:00 Mitra Bakhtiari Hamid Mozafari Khalil Karimzadeh Asl Behzad Sani Mehdi Mirza <p>The present study was carried out to assess the effects of bio-organic and inorganic fertilizers on plant nutrients, essential oil composition and antioxidant capacity of Satureja macrantha L. The experiment included nine treatments viz., NPK (50:25:25 kg ha−1), Vermicompost (VC) (5 t ha–1), NPK +VC, Thiobacillus (T), T+VC, T+sulfur (S) (250 kg ha−1), T+S 500 kg ha−1, Glomus mosseae, and control (untreated plants). The results showed the highest Essential Oil (EO) content and yield were respectively observed in plants treated with the combination of VC and NPK. Total Phenol Content (TPC) in first-year plants treated with VC and second-year plants under NPK+VC were higher than other experimental plants. Total Flavonoid Content (TFC) in second-year plants under the combination of NPK was greater than other plants. N content in first year plants treated with NPK fertilizer / combination of NPK and VC was higher compared to other experimental plants. The highest P content was observed in the NPK application in first year. Gas Chromatography—Mass Spectrometry (GC/MS) analyses revealed that the main constitutes of S. macrantha EO were p- Cymene (16.30-34.64%), γ-terpinene (15.46-33.6%), and Thymol (14.82-43.09%), which had different responses to sampling time and fertilizer treatments.</p> 2020-07-03T13:06:20+00:00 ##submission.copyrightStatement## Statistical analysis about diffusion of exercise addiction in Sicily 2020-07-06T21:09:39+00:00 Fabio Venturella Anastasia Valentina Liga Marco Giammanco Francesca Catania Giulia Cancellieri <p>Exercise Addiction (EA) is a behavioural dysfunction marked by uncontrolled compulsion towards all kinds of physical activity. With the purpose of understanding the epidemiology of this steadily increasing phenomenon, we have done a statistical analysis about some data obtained through the administration of an onlinequestionnaire (Google forms): the same one was also converted into paper-questionnaire and then it has been administered in many gyms in Palermo and Trapani (Sicily-Italy). The sample examined consists of 976 people aged between 14 and 65 (47.3% of them are women and 52.7% are men). For 53.8% of analysed people, physical activity is one of the most important things of their life. In fact, 18% of them declared to have conflicts with family or friends, because of the excessive hours dedicated to sport. Moreover, 86.2% of them pointed out that physical activity is a mood-booster. Probably for this reason, 49.6% of them claimed that they have drastically increased the number of hours devoted to physical activities in last years. Now, the most impressive statistic is the following one: 23.5% of surveyed declared that they get restless and nervous if they are unable to perform the training. Thanks to the last data, a clear symptom of craving emerged. It is very complicated to make a diagnosis of EA: however, through these questionnaires, the data indicate the presence of behaviours related to the Exercise Addiction. For this reason, it is necessary to act with preventive and information works in order to stem this not fully known phenomenon as much as possible.</p> 2020-07-06T14:48:24+00:00 ##submission.copyrightStatement##