Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale 2019-08-25T17:45:16+02:00 Francesca Baccino Open Journal Systems <p>The <strong>Journal of Biological Research – Bollettino della <a href="" target="_blank" rel="noopener">Società Italiana di Biologia Sperimentale</a></strong> is one of the oldest journals in Biology. Founded in 1925 the journal is available in <a href="[jour]" target="_blank" rel="noopener">Medline</a> until 2001 and <a href=";origin=resultslist" target="_blank" rel="noopener">Scopus</a> since 2014. The <strong>Journal of Biological Research – Bollettino della&nbsp;<a href="" target="_blank" rel="noopener">Società Italiana di Biologia Sperimentale</a></strong>&nbsp;is an online-only peer-reviewed journal which welcomes papers dealing with any aspect of experimental biology. Papers concerning clinical topics can be accepted only if they include experimental laboratory data. Original communications, review articles or short communications may be submitted.</p> Esterase profiling and molecular identification of yeasts isolated from different environmental samples from Morocco 2019-08-25T17:45:16+02:00 Lamya El Aamri Fabio Scordino Cinzia Barresi Orazio Romeo Giuseppe Criseo Majida Hafidi <p>One hundred and six fungal strains were isolated from different environmental samples (fresh olive oil cake, exhausted olive oil cake, black olive, rancid butter samples, rotten bread and Roquefort) collected from the region of Meknes, Morocco (coordinates: 33°53′42″N 5°33′17″W). Yeast isolates were tested for their esterase production ability using a qualitative method based on Tween agar plate assay. Enzymatic activity was also confirmed by a quantitative method relying on esterase production in liquid medium (6 days at 28°C with shaking). Molecular characterization of the selected esterase-producing yeasts was performed by sequencing the internal transcribed spacer 1 (ITS1), 5.8S and ITS2 region of the rDNA. A total of five different species were identified in this study: <em>Candida aaseri</em> (LE.26, LE.27 and LE.31 strains), <em>Wickerhamomyces anomalus</em> (LE.106, LE.112 and LE.115 strains), <em>Metschnikowia rancensis</em> (LE.153 strain), <em>Pichia</em> sp., (LE.102) and <em>Rhodotorula</em> <em>mucilaginosa</em> (LE.171 strain). Esterase production in <em>C. aaseri</em> and <em>W. anomalus</em> was found to be strain-dependent while for <em>M. rancensis</em>, this represents the first study reporting this species as an esterase producer.</p> 2019-08-02T12:50:07+02:00 ##submission.copyrightStatement## The effectivity of topical mangosteen pericarp extract cream on wound healing in Swiss albino mice 2019-08-25T17:45:14+02:00 Hilda Brigitta Sombolayuk Khairuddin Djawad Siswanto Wahab Upik A. Miskad Gemini Alam Ilhamjaya Pattelongi <p>Wound healing is a complex physiological process consisting of four phases: coagulation, inflammation, proliferation and migration, and remodeling, each with distinct characteristics. Studies have suggested that mangosteen pericarp extract (MPE) may accelerate wound healing. However, the mechanism is not fully understood. This study aims to evaluate the effect of MPE cream in various concentrations pericarp in acute wound healing of albino mice, both histologically and macroscopically. Thirty-two healthy female Swiss albino mice, aged 6-9 weeks, weight 20-30 g, were included in this study. The samples were randomly divided into eight groups each consisting of 4 mice. The first four groups were treated with MPE cream 5%, 10%, and 20%, and a control group that was given no medication, respectively, and were sacrificed after three days. The other four groups received the same application and were sacrificed after 8 days. Wound bed diameter was measured and biopsy from the skin lesion was performed for histopathologic examination. Mann-Whitney test was used to analyze the diameter of the wound bed and histopathological findings of granulation tissue formation, reepithelialization, and inflammation, with P&lt;0.05 considered as significant. MPE cream significantly improved wound healing by increasing granulation tissue formation, and reepithelialization. In addition, MPE cream application was also shown to decrease the number of inflammatory cells, particularly in 5% and 10% concentrations, both in the 3-day and 8-day groups. MPE cream application can accelerate wound healing and thus it can be used in acute wound treatment.</p> 2019-08-02T14:34:47+02:00 ##submission.copyrightStatement##