TY - JOUR AU - Yusuf, Muhammad AU - Usdyana Attahmid, Nur Fitriani PY - 2021/08/25 Y2 - 2024/03/28 TI - GC-MS analysis and antibacterial activity of the Sea cucumber (Muelleria lecanora) extract JF - Journal of Biological Research - Bollettino della Società Italiana di Biologia Sperimentale JA - J Biol Res VL - 94 IS - 2 SE - Original Articles DO - 10.4081/jbr.2021.9765 UR - https://www.pagepressjournals.org/jbr/article/view/9765 SP - AB - <p>Sea cucumbers are marine invertebrates commonly found in benthic areas and deep seas. On a global scale, they have a high commercial value with an increased level of production and trade. This study aims to analyze the bioactive compound in Sea cucumber (<em>Muelleria lecanora</em>) using the Ultrasound-Assisted Extraction Method (UAE) and Gas Chromatography- MassSpectrometry (GC-MS). Furthermore, it identifies the antibacterial activityin microorganisms <em>Salmonella</em>, <em>Escherichia coli,</em> and <em>Staphylococcus aureus</em>. The bioactive compounds were extracted using methanol, acetone, and n-hexane solvent and were separated by ultrasound-assisted extraction. In the initial stage, phytochemicals were screened using Gas Chromatography-MassSpectrometry (GC-MS). Disc diffusion method was then used to determine theantibacterial activity against <em>Salmonella</em>, <em>Staphylococcus aureus</em>, and <em>Escherichia coli.</em> The results showed that methanol extract is more suitable for extracting bioactive compounds of <em>Muelleria lecanora</em> than acetone and n-hexane. Meanwhile, acetone solvents are more suitable for the production of flavonoid and steroid compounds than Mulleria lecanora samples. Heneicosane compounds that function as a new antiproliferative for inhibition of tumor and cancerous cells are produced from n-hexane. The antibacterial activity of acetone, methanol and n-hexane extract determined by diffusion assay was effective against <em>Staphylococcus aureus</em> and <em>Salmonella</em> but ineffective against <em>Escherichia coli</em>. GC-MS results showed that the major constituents obtained were steroid and flavanoid. From this study, Sea cucumber extract can be considered a healthy nutrientin food and pharmaceutical products.</p> ER -