Growth, yield, nutrients uptake and anatomical properties of direct seeding and transplanting maize (Zea mays L.) plants under arbuscular mycorrhizal fungi and water stress

  • Saeed Rezazadeh Department of Agronomy and Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran, Islamic Republic of.
  • Mohammadnabi Ilkaee | Department of Agronomy and Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran, Islamic Republic of.
  • Fayaz Aghayari Department of Agronomy and Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran, Islamic Republic of.
  • Farzad Paknejad Department of Agronomy and Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran, Islamic Republic of.
  • Mehdi Rezaee Department of Agronomy and Plant Breeding, Karaj Branch, Islamic Azad University, Karaj, Iran, Islamic Republic of.


The management of cultivation technology and fertilizer application may adjust adverse effects of abiotic stresses such as water deficit on agricultural products. Therefore, a field experiment was carried out on growth, yield, nutrient uptake and anatomical properties of maize under three water regimes (well-watered, moderate stress and severe stress as 25%, 50% and 75% soil moisture depletion), two cultivations methods (direct seeding and transplanting), and two Arbuscular Mycorrhizal Fungi (AMF) levels (inoculated with Glomus mosae and uninoculated). The results showed that in plants under moderate water stress, the AMF inoculation percent was significantly higher than those under well-watered and sever stress condition. Inoculation percent in direct seeding was lower than transplanting. Transplanting plants had higher biological and kernel yield compared to direct seeding plants. Water stress reduced the total chlorophyll (Chl) content. Transplanting had greater Chl content in comparison with direct seeding. In all irrigation regimes, transplanting significantly increased N content. In direct seeding, the highest P content was observed in moderate stress and uninoculated plants. Stomatal density increased under water stress, but stomatal size decreased. Plants under severe water stress showed increased stomatal density compared with well waterbed conditions. In addition, severe water stress enhanced the UCT compared to well-watered condition. This study suggests the use of transplanting with AMF application to cope with the adverse effects of severe water stress on maize.



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Ruiz‐Lozano JM, Aroca R, Zamarreño ÁM, et al. Arbuscular mycorrhizal symbiosis induces strigolactone biosynthesis under drought and improves drought tolerance in lettuce and tomato. Plant Cell Environ 2016;39:441-52. DOI:

Golldack D, Li C, Mohan H, Probst N. Tolerance to drought and salt stress in plants: unraveling the signaling networks. Front Plant Sci 2014;5:151-8. DOI:

Farooq, M, Wahid, A, Kobayashi, N, et al. Plant drought stress: effects, mechanisms and management. In Sustain Agr 2009;153-88. Springer, Dordrecht. DOI:

Grümberg, BC, Urcelay, C, Shroeder, MA, et al. The role of inoculum identity in drought stress mitigation by arbuscular mycorrhizal fungi in soybean. Biol Fert Soil 2015;51:1-10. DOI:

Ortiz N, Armada E, Duque E, et al. Contribution of arbuscular mycorrhizal fungi and/or bacteria to enhancing plant drought tolerance under natural soil conditions: effectiveness of autochthonous or allochthonous strains. J Plant physiol 2015;174:87-96. DOI:

Lampayan RM, Faronilo JE, Tuong TP, et al. Effects of seedbed management and delayed transplanting of rice seedlings on crop performance, grain yield, and water productivity. Field Crop Res 2015;183:303-14. DOI:

Augé RM, Toler HD, Saxton AM. Arbuscular mycorrhizal symbiosis alters stomatal conductance of host plants more under drought than under amply watered conditions: a meta-analysis. Mycorrhiza 2015;25:13-24. DOI:

Symanczik S, Lehmann MF, Wiemken A, et al. Effects of two contrasted arbuscular mycorrhizal fungal isolates on nutrient uptake by Sorghum bicolor under drought. Mycorrhiza 2018;28:779-85. DOI:

Birnbaum, SJ, Poole JM, Williamson PS. Reintroduction of star cactus Astrophytumasterias by seed sowing and seedling transplanting, Las Estrellas Preserve, Texas, USA. Cons Evid 2011;8:43-52.

Sánchez JM, López-Urrea R, Rubio E, & Caselles V. Determining water use of sorghum from two-source energy balance and radiometric temperatures. Hydrol Earth System Sci 2011;15:3061-70. DOI:

Zadworny M, Eissenstat, DM. Contrasting the morphology, anatomy and fungal colonization of new pioneer and fibrous roots. New Phytol 2011;190:213-21. DOI:

Arnon DI. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 1949;24:1-5. DOI:

Maathuis, FJ. Plant mineral nutrients: Methods and protocols. New Delhi, India: Humana Press: 2013. DOI:

Khosropour E, Attarod P, Shirvan A, et al. Response of Platanus orientalis leaves to urban pollution by heavy metals. J For Res 2019;30:1437-45. DOI:

Zhao R, Guo W, Bi N, Guo J. Arbuscular mycorrhizal fungi affect the growth, nutrient uptake and water status of maize (Zeamays L.) grown in two types of coal mine spoils under drought stress. Appl Soil Ecol 2015;88:41-9. DOI:

El-Mesbahi MN, Azcón R, Ruiz-Lozano JM, Aroca R. Plant potassium content modifies the effects of arbuscular mycorrhizal symbiosis on root hydraulic properties in maize plants. Mycorrhiza 2012;22:555-64. DOI:

Gholamhoseini M, Ghalavand A, Dolatabadian A. Effects of arbuscular mycorrhizal inoculation on growth, yield, nutrient uptake and irrigation water productivity of sunflowers grown under drought stress. Agr Water Manage 2013;117:106-14. DOI:

Gill G, Humphreys E, Kukal SS, Walia US. Effect of water management on dry seeded and puddled transplanted rice. Part 1: Crop performance. Field Crop Res 2011;120:112-22. DOI:

Lakra N, Nutan KK, Das P.A nuclear-localized histone-gene binding protein from rice (OsHBP1b) functions in salinity and drought stress tolerance by maintaining chlorophyll content and improving the antioxidant machinery. J Plant Physiol 2015;176:36-46. DOI:

Shivakrishna P, Reddy KA, Rao DM. Effect of PEG-6000 imposed drought stress on RNA content, relative water content (RWC), and chlorophyll content in peanut leaves and roots. Saudi J Biol Sci 2018;25:285-9. DOI:

Elhindi KM, El-Din AS, Elgorban AM, 2017, The impact of arbuscular mycorrhizal fungi in mitigating salt-induced adverse effects in sweet basil (Ocimum basilicum L.). Saudi J Biol Sci 24:170-9. DOI:

Mohammadi M, Modarres-Sanavy SAM, Pirdashti H, et al. Arbuscular mycorrhizae alleviate water deficit stress and improve antioxidant response, more than nitrogen fixing bacteria or chemical fertilizer in the evening primrose. Rhizosphere 2019;9:76-89. DOI:

Deng M, Li P, Wang Z, et al. Drought and salinization stress induced by stand development alters mineral element cycling in a larch plantation. J Geophys Res Biogeosci 2021;126:e2020JG005906. DOI:

Hijikata N, Murase M, Tani C. Polyphosphate has a central role in the rapid and massive accumulation of phosphorus in extraradical mycelium of an arbuscular mycorrhizal fungus. New Phytol 2010;186:285-9. DOI:

Steinthorsdottir M, Vajda, Pole M. Significant transient pCO2 perturbation at the New Zealand Oligocene-Miocene transition recorded by fossil plant stomata. Palaeogeogr Palaeoclimatol Palaeoecol 2019;515:152-61. DOI:

YangH, Wang G. Leaf stomatal densities and distribution in triticum aestivum under drought and CO_ (2) enrichment. Acta Phytoecol Sinica 2001;25:312-6.

Gan Y, Zhou L, Shen ZJ, et al. Stomatal clustering, a new marker for environmental perception and adaptation in terrestrial plants. Bot Stud 2010;51:1-7.

Haworth M, Scutt CP, Douthe C, et al. Allocation of the epidermis to stomata relates to stomatal physiological control: stomatal factors involved in the evolutionary diversification of the angiosperms and development of amphistomaty. Environ Exp Bot 2018;151:55-63. DOI:

Quarrie, SA, Jones HG. Genotypic variation in leaf water potential, stomatal conductance and abscisic acid concentration in spring wheat subjected to artificial drought stress. Ann Bot 1979;44:323-32. DOI:

Xu Z, Zhou G. Responses of leaf stomatal density to water status and its relationship with photosynthesis in a grass. J Exp Bot 2008;59:3317-25. DOI:

Zhang H, Wang X, Wang S. A study on stomatal traits of Platanusacerifolia under urban stress. J Fudan University Nat Sci 2004;43:651-6.

Comas LH, Trout TJ, DeJonge KC, et al. Water productivity under strategic growth stage-based deficit irrigation in maize. Agr Water Manage2019;212:433-40. DOI:

Fontana M, Labrecque M, Collin A, Bélanger N. Stomatal distribution patterns change according to leaf development and leaf water status in Salix miyabeana. Plant Growth Regul 2017;81:63-70. DOI:

Original Articles
Cultivation method, fertilizer management, biological yield, stomatal density
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How to Cite
Rezazadeh, S., Ilkaee, M., Aghayari, F., Paknejad, F., & Rezaee, M. (2021). Growth, yield, nutrients uptake and anatomical properties of direct seeding and transplanting maize (<em>Zea mays</em&gt; L.) plants under arbuscular mycorrhizal fungi and water stress. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 94(1).