Comparative study of estrogen receptor α, β mRNA expressions of endometriosis and normal endometrium in women and analysis of potential synthetic anti-estrogens in silico


Submitted: May 8, 2018
Accepted: November 5, 2018
Published: November 28, 2018
Abstract Views: 954
PDF: 387
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Authors

  • Eldafira Eldafira Department of Biology, Faculty of Mathematics and Natural Sciences; Department of Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
  • Abinawanto Abinawanto Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Jakarta, Indonesia.
  • Luthfiralda Sjahfirdi Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Jakarta, Indonesia.
  • Asmarinah Asmarinah Department of Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
  • Purnomo Soeharso Department of Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
  • Muharam Muharam Department of Obstetrics and Gynecology, RSCM, Jakarta, Indonesia.
  • Vivitri D. Prasasty Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta, Indonesia.
  • Dwi A. Pujianto Department of Biology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.

Endometriosis is a multifactorial disease in which genetic and environmental factors interact causing its pathogenesis. The aim of this study was to investigate the expression pattern of estrogen receptor α (ERα) and β (ERβ) in endometriosis patients compared to normal endometrioum (n=18) as a control by using Quantitative Real Time PCR method. Moreover, we also measured serum estradiol levels of endometriosis patients in the proliferation phase of the menstrual cycle using the enzyme-linked immunosorbent assay method. The mRNA expression of ERβ was significantly higher in the endometriosis group compared to control, and the result of t-test showed that were significantly different (P<0.05). Overexpression of ERβ in endometriosis was likely to have other significant important impacts in the pathology of endometriosis that allowed ERβ to stimulate prostaglandin production in endometriosis tissue and cells. Estradiol content did not correlate with the ERα expression, and it is weakly correlated with ERβ mRNA expression. Molecular docking analysis showed that ERα and ERβ have different binding interactions with synthetic antiestrogens, whereas the best inhibitor was Ral2 to ERα and Aco1 to ERβ. Thus, both inhibitors could be used as leads in further investigation of ERα, ERβ inhibitory activities in vitro and in vivo.


Supporting Agencies

Department of Biology of Faculty of Mathematics and Life Sciences, Faculty of Medicine, University of Indonesia.

Eldafira, E., Abinawanto, A., Sjahfirdi, L., Asmarinah, A., Soeharso, P., Muharam, M., Prasasty, V. D., & Pujianto, D. A. (2018). Comparative study of estrogen receptor α, β mRNA expressions of endometriosis and normal endometrium in women and analysis of potential synthetic anti-estrogens in silico. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 91(2). https://doi.org/10.4081/jbr.2018.7550

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