Molecular mechanisms mediating the neuroproyective effects of quinacrine and minocycline on cell death induced by the prion protein fragment 90-231 (hPrP90-231)

V. Villa; A. Corsaro; S. Thellung; A. Simi; M. Nizzari; M. Tonelli; V. Boido; A. Aceto; T. Florio

doi:10.4081/jbr.2011.4689

Molecular mechanisms mediating the neuroproyective effects of quinacrine and minocycline on cell death induced by the prion protein fragment 90-231 (hPrP90-231)

https://doi.org/10.4081/jbr.2011.4689

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Vol. 84 No. 1 (2011)

Submitted: September 5, 2014
Accepted: September 5, 2014

Published: January 30, 2011

prion protein, quinacrine, minocycline

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The effects of quinacrine and minocycline on the toxicity induced by hPrP90-231 were studied. By mild thermal denaturation, hPrP90-231 can be converted in a toxic PrP^Sc-like structure affecting the survival of SH-SY5Y cells. Quinacrine and minocycline prevented hPrP90-231-induced toxicity interfering with different mechanisms: protective effects of quinacrine are mediated by the binding to the fragment that abolished hPrP90-231 structural changes and cell internalization, whereas, minocycline reverted MAP kinase neurotoxic signaling exerted by the prion fragment.

Villa, V., Corsaro, A., Thellung, S., Simi, A., Nizzari, M., Tonelli, M., Boido, V., Aceto, A., & Florio, T. (2011). Molecular mechanisms mediating the neuroproyective effects of quinacrine and minocycline on cell death induced by the prion protein fragment 90-231 (hPrP90-231). Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 84(1). https://doi.org/10.4081/jbr.2011.4689