Cardiac regeneration by pharmacologically active microcarriers releasing growth factors and/or transporting adipose-derived stem cells

  • Monia Savi | monia.savi@unipr.it Department of Biosciences, University of Parma, Italy.
  • Leonardo Bocchi National Institute of Cardiovascular Research – INRC, University of Bologna, Italy.
  • Emanuela Fiumana Department of Biochemistry, University of Bologna, Italy.
  • Caterina Frati Department of Biomedical, Biotechnological and Translational Sciences, University of Parma, Italy.
  • Francesca Bonafé Department of Biochemistry, University of Bologna, Italy.
  • Stefano Cavalli Department of Biomedical, Biotechnological and Translational Sciences, University of Parma, Italy.
  • Paolo Giovanni Morselli Department of Specialized Surgery and Anesthetic Sciences, University of Bologna, Italy.
  • Jean-Pierre Karam INSERM U646, University of Angers, France.
  • Claudia Montero-Menei INSERM U646, University of Angers, France.
  • Claudio Marcello Caldarera Department of Biochemistry, University of Bologna, Italy.
  • Carlo Guarnieri Department of Biochemistry, University of Bologna, Italy.
  • Claudio Muscari Department of Biochemistry, University of Bologna, Italy.
  • Donatella Stilli National Institute of Cardiovascular Research – INRC, University of Bologna, Italy.
  • Federico Quaini National Institute of Cardiovascular Research – INRC, University of Bologna, Italy.
  • Ezio Musso National Institute of Cardiovascular Research – INRC, University of Bologna, Italy.

Abstract

We tested the hypothesis that cardiac regeneration through local delivery of adipose-derived stem cells (ASCs), activation of resident cardiac stem cells via growth factors (GFs) [hepatocyte growth factor (HGF) and insulin-like growth factor 1 (IGF-1):GFs] or both, are improved by pharmacologically active microcarriers (PAMs) interacting with cells/molecules conveyed on their surface. Rats with one-month old myocardial infarction were treated with ASCs, ASCs+PAMs, GF-releasing PAMs, ASCs+GF-releasing PAMs or vehicle. Two weeks later, hemodynamic function and inducibility of ventricular arrhythmias (VAs) were assessed. Eventually, the hearts were subjected to anatomical and immunohistochemical analyses. A significant ASCs engraftment and the largest improvement in cardiac mechanics occurred in ASC+GF-releasing PAM rats which by contrast were more vulnerable to VAs. Thus, PAMs may improve cell/GF-based cardiac regeneration although caution should be paid on the electrophysiological impact of their physical interaction with the myocardium.

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Published
2014-01-10
Keywords:
myocardial infarction, stem cell therapy, adipose-derived stem cells, growth factor, scaffold
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How to Cite
Savi, M., Bocchi, L., Fiumana, E., Frati, C., Bonafé, F., Cavalli, S., Morselli, P. G., Karam, J.-P., Montero-Menei, C., Caldarera, C. M., Guarnieri, C., Muscari, C., Stilli, D., Quaini, F., & Musso, E. (2014). Cardiac regeneration by pharmacologically active microcarriers releasing growth factors and/or transporting adipose-derived stem cells. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 87(1). https://doi.org/10.4081/jbr.2014.2141