https://doi.org/10.4081/jbr.2023.10803

Serum Calprotectin and B-cell activating factor are potential biomarkers for Helicobacter pylori infection

Vol. 96 No. 1 (2023)
Submitted: August 16, 2022
Accepted: March 2, 2023
Published: March 31, 2023
Helicobacter pylori infection, inflammatory markers, Calprotectin, B-cell activating factor
Abstract Views: 1543
PDF: 293
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Authors

Akam Jasim Mustafa
akam.mustafa@soran.edu.iq
https://orcid.org/0000-0002-3759-3132
Department of Chemistry, Faculty of Science, Soran University, Soran, Kurdistan Region, Iraq.
Hazhar M. Balaky
https://orcid.org/0000-0002-4579-2617
Mergasor Technical Institute, Erbil Polytechnic University, Erbil, Kurdistan Region, Iraq.
Parween Abdulsamad Ismail
https://orcid.org/0000-0002-2410-4889
Department of Chemistry, College of Education, University of Salahaddin, Erbil, Kurdistan Region, Iraq.
Hawdang Othman Abdalla
https://orcid.org/0000-0002-4001-9545
Department of Chemistry, Faculty of Science, Soran University, Soran, Kurdistan Region, Iraq.
Khawla Mahruf Muhammed
Department of Chemistry, Faculty of Science, Soran University, Soran, Kurdistan Region, Iraq.

Humans always mount a robust immune response to the bacterial infection caused by Helicobacter pylori, which causes various gastrointestinal tract infections. Calprotectin (CALP) and B-Cell Activating Factor (BAFF) are inflammatory biomarkers having a role in the gastrointestinal neutrophilic response to bacterial infection. The study was designed to assess serum CALP and BAFF as inflammatory biomarkers in H. pylori infection and peptic ulcer patients. The current study comprised 112 people, including 62 H. pylori-infected patients (34 men and 28 women) who were clinically diagnosed with H. pylori infection via testing positive for the H. pylori stool antigen test; they were compared to a control group of 50 healthy people (34 men and 16 women) who were age and gender- matched to H. pylori-infected patients. The serum level of CALP and BAFF were assayed using the ELISA technique. The biochemical parameters were statistically compared between patients and controls by unpaired Man-Whitney U t-test and Receiver Operating Characteristic (ROC) curve analysis. There was a significant elevation of serum CALP in H. pylori-infected patients [116.4(120.7), p=0.0132] in comparison to healthy controls [99.50(115.8)]. Similarly, there was a significant elevation of serum BAFF concentration in H. pylori-infected patients [485.7(367.1), p=0.0014] in comparison to healthy controls [444.5(513.0)]. The ROC curve analysis suggests serum CALP and BAFF as reasonable inflammatory biomarkers for H. pylori infection with statistically significant (p=0.0135, p=0.0015) area under the ROC curve of (0.6361, 0.6748), respectively. CALP and BAFF are potent inflammatory biomarkers involved in the development and etiology of H. pylori infection. Serum CALP and BAFF levels could be used as biomarkers for chronic inflammation induced by H. pylori. CALP and BAFF biomarkers can be combined to diagnose and predict the prognosis of H. pylori infection.

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How to Cite

Mustafa, A. J., Balaky, H. M., Ismail, P. A., Abdalla, H. O., & Muhammed, K. M. (2023). Serum Calprotectin and B-cell activating factor are potential biomarkers for <i>Helicobacter pylori</i> infection. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 96(1). https://doi.org/10.4081/jbr.2023.10803
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