Effects of injection speed of test samples on the mouse bioassay for paralytic shellfish poisoning toxins


Submitted: 25 February 2013
Accepted: 2 April 2013
Published: 17 June 2013
Abstract Views: 832
PDF - FULL TEXT IN ENG: 1174
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Authors

  • Hodaka Suzuki Division of Biomedical Food Research, National Institute of Health Sciences, Tokyo, Japan.
  • Kenji Machii Division of Biomedical Food Research, National Institute of Health Sciences, Tokyo, Japan.
The mouse bioassay has been used as the official method for paralytic shellfish poisoning toxins detection in Japan since 1980. However, differences in the results of this assay, when performed by different investigators, have been noted despite the use of the same sample. This study was performed to examine the effect of the injection speed, a hypothetical cause of such differences, on the death time of mice. Speed-controlled injection of the toxin (at 12, 6, 3, and 1.5 mL/min) into mice was performed using a syringe pump, and the death times of mice were measured. No statistically significant differences were found among the groups, even between fast injection (5 s) and very slow injection (40 s), indicating that the injection speed may not be the crucial factor for this assay.

Hodaka Suzuki, Division of Biomedical Food Research, National Institute of Health Sciences, Tokyo
Division of Biomedical Food Research
Kenji Machii, Division of Biomedical Food Research, National Institute of Health Sciences, Tokyo
Division of Biomedical Food Research

Supporting Agencies

the Ministry of Health, Labour and Welfare of Japan

1.
Suzuki H, Machii K. Effects of injection speed of test samples on the mouse bioassay for paralytic shellfish poisoning toxins. Ital J Food Safety [Internet]. 2013 Jun. 17 [cited 2024 Mar. 28];2(2):e21. Available from: https://www.pagepressjournals.org/ijfs/article/view/ijfs.2013.e21

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