IDENTIFICATION OF E. COLI O157 IN A BOVINE MILK FARM BY MULTIPLEX REAL-TIME PCR


Submitted: 4 January 2013
Accepted: 4 January 2013
Published: 2 August 2012
Abstract Views: 850
PDF: 1069
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

  • A. Petruzzelli IZSUM-Istituto Zooprofilattico Sperimentale Umbria e Marche, Italy, Italy.
  • G. Amagliani Dipartimento di Scienze Biomolecolari, Università di Urbino “Carlo Bo”, Urbino, Italy, Italy.
  • M. Foglini IZSUM-Istituto Zooprofilattico Sperimentale Umbria e Marche, Italy, Italy.
  • C. Bartolini IZSUM-Istituto Zooprofilattico Sperimentale Umbria e Marche, Italy, Italy.
  • F. Agnetti IZSUM-Istituto Zooprofilattico Sperimentale Umbria e Marche, Italy, Italy.
  • C. Magistrali IZSUM-Istituto Zooprofilattico Sperimentale Umbria e Marche, Italy, Italy.
  • E. Omiccioli Diatheva, Fano, Italy, Italy.
  • G. Brandi Dipartimento di Scienze Biomolecolari, Università di Urbino “Carlo Bo”, Urbino, Italy, Italy.
  • F. Tonucci IZSUM-Istituto Zooprofilattico Sperimentale Umbria e Marche, Italy., Italy.
Law provisions about direct sell of raw bovine milk require VTEC O157 monitoring in bovine milk farms (milk and faeces). It has been showed that culture-based methods used for this scope, besides being cumbersome and time-consuming, may be also less sensitive, compared to molecular approaches. In this study, a multiplex Real-Time PCR, able to identify VTEC O157, Salmonella spp and Listeria monocytogenes, has been used to analyse milk, filter, sewage and stool samples from a milk farm, in comparison with standard OIE methods. The performances of the molecular protocol have been preliminary assessed with lyophilized samples from proficiency testing VLA, showing 100% accordance. Results from field samples indicated the absence of the pathogen in milk, and the higher sensitivity of Real-Time PCR with other matrices, suggesting its potential use for fast VTEC O157 identification.

1.
Petruzzelli A, Amagliani G, Foglini M, Bartolini C, Agnetti F, Magistrali C, Omiccioli E, Brandi G, Tonucci F. IDENTIFICATION OF E. COLI O157 IN A BOVINE MILK FARM BY MULTIPLEX REAL-TIME PCR. Ital J Food Safety [Internet]. 2012 Aug. 2 [cited 2024 Mar. 29];1(4):105-6. Available from: https://www.pagepressjournals.org/ijfs/article/view/ijfs.2012.4.105

Downloads

Download data is not yet available.

Citations