MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY


Submitted: 4 January 2013
Accepted: 4 January 2013
Published: 8 January 2011
Abstract Views: 564
PDF: 1472
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Authors

  • A. Petruzzelli Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia, Italy.
  • F. Ciarrocchi Dipartimento di Scienze Biomolecolari, Università degli studi di Urbino “Carlo Bo” – Urbino, Italy.
  • M. Foglini Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia, Italy.
  • S. Fisichella Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia, Italy.
  • C. Sebastiani Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia, Italy.
  • W. Baffone Dipartimento di Scienze Biomolecolari, Università degli studi di Urbino “Carlo Bo” – Urbino., Italy.
  • S. Santarelli Dipartimento SAIFET, Università Politecnica delle Marche – Ancona, Italy.
  • F. Tonucci Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia., Italy.
  • G. Blasi Istituto Zooprofilattico Sperimentale dell’Umbria e delle Marche – Perugia., Italy.
The present study reports the results of a preliminary characterization of the bacterial population of Ciauscolo, a typical Italian fermented sausage, traditionally manufactured in Marche region. The bacterial community involved in Ciauscolo fermentation was investigated using both molecular and culturebased methods. The estimation of genotypic intra-species variation of the autochthonous bacteria isolated was also evaluated by using randomly amplified polymorphic DNA (RAPD) analysis and unweighted pairgroup method with arithmetic averages (UPGMA) cluster analysis. Our findings revealed an high diversity of the autochthonous bacterial population investigated, both at species and strain level.

1.
Petruzzelli A, Ciarrocchi F, Foglini M, Fisichella S, Sebastiani C, Baffone W, Santarelli S, Tonucci F, Blasi G. MOLECULAR IDENTIFICATION AND GENOTYPING OF CIAUSCOLO AUTOCHTHONOUS MICROFLORA: PRELIMINARY STUDY. Ital J Food Safety [Internet]. 2011 Jan. 8 [cited 2024 Mar. 28];1(1zero):287-8. Available from: https://www.pagepressjournals.org/ijfs/article/view/ijfs.2011.1S.287

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