DEVELOPMENT OF A PCR-RFLP METOD FOR THE IDENTIFICATION OF SIX SPECIES BELONGING TO THE GENUS LOPHIUS


Submitted: 13 February 2013
Accepted: 13 February 2013
Published: 13 December 2009
Abstract Views: 1390
PDF: 1094
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Authors

  • A. Armani Dipartimento di Patologia Animale, Profilassi ed Igiene degli Alimenti, Università di Pisa, .
  • L. Castigliego Dipartimento di Patologia Animale, Profilassi ed Igiene degli Alimenti, Università di Pisa, .
  • A. Guidi Dipartimento di Patologia Animale, Profilassi ed Igiene degli Alimenti, Università di Pisa, .
  • G. Gandini Ministero della Salute, PIF Bologna, .
  • P. Manzoni
  • F. Susini Istituto Zooprofilattico Lazio e Toscana, laboratorio di Ittiopatologia, .
  • D. Gianfaldoni Dipartimento di Patologia Animale, Profilassi ed Igiene degli Alimenti, Università di Pisa, .
Nowadays, six of the seven species belonging to the Genus Lophius have an important commercial value in the national and international markets. Usually they are sold beheaded and for this reason they are called tails. This kind of preparation is a limit for the specie-identification by means of the morphological characteristics. The mitochondrial cytochrome b (Cyt b) gene is considered a useful genetic marker to identify fish species. In this work, after obtaining the Cyt b complete sequence of the Lophius species that were missed in the databases, we set up a method based on PCR-RFLP able to identify the six species of Lophius with a commercial denomination in the Italian market.

1.
Armani A, Castigliego L, Guidi A, Gandini G, Manzoni P, Susini F, Gianfaldoni D. DEVELOPMENT OF A PCR-RFLP METOD FOR THE IDENTIFICATION OF SIX SPECIES BELONGING TO THE GENUS LOPHIUS. Ital J Food Safety [Internet]. 2009 Dec. 13 [cited 2024 Apr. 20];1(6):63-7. Available from: https://www.pagepressjournals.org/ijfs/article/view/ijfs.2009.6.63

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