https://doi.org/10.4081/hls.2023.11766
The effect of Vigna unguiculata on the estrogen receptor-α expression and the endometrial thickness in rats treated with depot medroxyprogesterone acetate (DMPA)
Submitted: 11 September 2023
Accepted: 18 October 2023
Published: 5 December 2023
Accepted: 18 October 2023
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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Depot medroxyprogesterone acetate (DMPA) is a contraceptive method that prevents ovulation and reduces endometrial thickness. This study aimed to investigate the influence of Vigna unguiculata (VU) on estrogen receptor-α expression and endometrial thickness in rats subjected to DMPA treatment. The research utilized a true experimental design involving 25 female Wistar rats divided into 5 experimental groups. The DMPA+VU experiment lasted for 4 weeks, and data were analyzed using a Complete Randomized Design. Estrogen receptor-α expression in the endometrium was assessed through immunohistochemical techniques, and endometrial thickness was determined via Hematoxylin-eosin (HE) staining, observed under dot slide microscopes (Olympus XC10) at 400× magnification. The study found that estrogen receptor-α expression and endometrial thickness were significantly higher in both the DMPA+VU2 and DMPA+VU3 groups compared to the DMPA group and the DMPA+VU1 group (p<0.05). DMPA treatment reduced estrogen receptor-α expression and endometrial thickness. However, the administration of Vigna unguiculata extracts at 2.5 mg/kg and 5 mg/kg led to an increase in estrogen receptor-α expression and endometrial thickness. The study implies that VU extract may have a positive impact on estrogen receptor-α expression and endometrial thickness in rats treated with depot medroxyprogesterone acetate.
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How to Cite
Fitrianingtyas, R., Christiana, I., Handayani, R., Anggraeni, E., Mauludiyah, Z., & Hidayati, S. (2023). The effect of <i>Vigna unguiculata</i> on the estrogen receptor-α expression and the endometrial thickness in rats treated with depot medroxyprogesterone acetate (DMPA). Healthcare in Low-Resource Settings, 12(1). https://doi.org/10.4081/hls.2023.11766
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