Peripheral axotomy-induced changes of motor function and histological structure of spinal anterior horn

  • Amir Raoofi Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Mohammad Amin Abdollahifar Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Abbas Aliaghaei Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Abbas Piryaei Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Fatemeh Hejazi Department of Polymer Engineering and Color Technology, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
  • Ensieh Sajadi Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Ali Rashidiani-Rashidabadi Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
  • Yousef Sadeghi | dr.ysadeghi@yahoo.com Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Abstract

The aim of this study was to evaluate changes of both peripheral motor function and histology of spinal anterior horn in adult rats after unilateral sciatectomy. Ten adult healthy rats served as control group, while in the ten rat experimental group the right sciatic nerve was severed. We followed-up nerve motor function using a sciatic function index and electromyography activity of the gastrocnemious muscle. The rats of the experimental group presented the expected gross locomotor deficit and leg muscle atrophy. At 12 weeks post sciatectomy, L4 and L5 spinal cord segments were removed from the twenty rats and were analysed by istological stereological methods. In the axotomized animals volume of the anterior horn and its content of motor neurons decreased, while the content of astrocytes increased (p < 0.05). Thus, in adult rats, beside the obvious peripheral nerve disfuction, the sciatic nerve axotomy have severe consequences on the soma of the injured motor neurons in the spinal anterior horn. All these quantitative analyses may be usefull to quantify changes occurring in adult animals after axotomy and eventual management to modify the final outcomes in peripheral nerve disorders.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.
Published
2019-01-17
Info
Issue
Section
Original Articles
Keywords:
Peripheral axotomy, motor function, anterior horn, stereology, degeneration
Statistics
  • Abstract views: 648

  • PDF: 246
  • HTML: 14
How to Cite
Raoofi, A., Abdollahifar, M., Aliaghaei, A., Piryaei, A., Hejazi, F., Sajadi, E., Rashidiani-Rashidabadi, A., & Sadeghi, Y. (2019). Peripheral axotomy-induced changes of motor function and histological structure of spinal anterior horn. European Journal of Translational Myology, 29(1). https://doi.org/10.4081/ejtm.2019.7945