https://doi.org/10.4081/ejtm.2018.7567

Culture conditions influence satellite cell activation and survival of single myofibers

Vol. 28 No. 2 (2018)
Submitted: 17 May 2018
Accepted: 24 May 2018
Published: 29 May 2018
satellite cells, proliferation, quiescence, growth factors, cell adhesion, myofibers, cell culture
Abstract Views: 1344
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APPENDIX: 316
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Authors

Alessandra Renzini
http://orcid.org/0000-0002-4265-7188
DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Italy.
Anna Benedetti
http://orcid.org/0000-0003-2450-3705
DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Italy.
Marina Bouché
http://orcid.org/0000-0002-0938-5360
DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Italy.
Leopoldo Silvestroni
http://orcid.org/0000-0002-2197-7019
Department of Fundamental and Basic Sciences for Engineering, Sapienza University of Rome, Italy.
Sergio Adamo
sergio.adamo@uniroma1.it
http://orcid.org/0000-0002-1409-0452
DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Italy.
Viviana Moresi
http://orcid.org/0000-0003-1912-0339
DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Italy.
Single myofiber isolation protocols allow to obtain an in vitro system in which the physical association between the myofiber and its stem cells, the satellite cells, is adequately preserved. This technique is an indispensable tool by which the muscle regeneration process can be recapitulated and studied in each specific phase, from satellite cell activation to proliferation, from differentiation to fusion. This study aims to clarify the effect of different culture conditions on single myofibers, their associated satellite cells, and the physiological behavior of the satellite cells upon long term culture. By direct observations of the cultures, we compared different experimental conditions and their effect on both satellite cell behavior and myofiber viability.

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Supporting Agencies

AFM Telethon, Sapienza University

How to Cite

Renzini, A., Benedetti, A., Bouché, M., Silvestroni, L., Adamo, S., & Moresi, V. (2018). Culture conditions influence satellite cell activation and survival of single myofibers. European Journal of Translational Myology, 28(2). https://doi.org/10.4081/ejtm.2018.7567

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