https://doi.org/10.4081/ejtm.2024.12249
Role of the ubiquitin-proteasome system in the sarcopenic-like phenotype induced by CCL5/RANTES
Submitted: 31 December 2023
Accepted: 1 February 2024
Published: 15 February 2024
Accepted: 1 February 2024
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Sarcopenia is characterized by reduced muscle strength and mass and a decline in muscle fiber diameter and amount of sarcomeric proteins. Sarcopenia involves the activation of the ubiquitin-proteasome system (UPS). MuRF-1 and atrogin-1 are E3 ubiquitin ligases belonging to UPS, leading to proteolysis mediated by the PSMB 5, 6, and 7 subunits of 20S proteasome. CCL5/RANTES induces a sarcopenic-like effect in muscle cells. The present work explored the impact of CCL5 on UPS components and the influence of UPS on its sarcopenic-like effect. We demonstrated that CCL5 increased MuRF-1 and atrogin-1 protein levels and mRNA levels of subunits PSMB 5, 6, and 7. We used the MG132 inhibitor to elucidate the role of the 20S proteasome in the CCL5-induced sarcopenic-like effect. This inhibitor prevented the decrease in troponin and MHC protein levels and partially prevented the reduction in the diameter of single-isolated FDB muscle fibers induced by CCL5. These findings indicate that CCL5 actively modulates the UPS. Moreover, our results show the direct participation of UPS in the sarcopenic-like phenotype induced by CCL5.
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How to Cite
Conejeros-Lillo, S., Aguirre, F., Cabrera, D., Simon, F., Peñailillo, L., & Cabello-Verrugio, C. (2024). Role of the ubiquitin-proteasome system in the sarcopenic-like phenotype induced by CCL5/RANTES. European Journal of Translational Myology, 34(1). https://doi.org/10.4081/ejtm.2024.12249
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