Influence of hCG on inducible nitric oxide synthase gene expression in ram testicular arteries

  • Maria Matteo | maria.matteo@unifg.it Operative Unit of Obstetric and Gynecology, Department of Surgical Sciences, University of Foggia, Italy.
  • Annalisa Rizzo Department of Animal Production, University of Bari, Italy.
  • Ettore Cicinelli 3rd Unit of Obstetrics and Gynecology, Department of Biomedical and Human Oncological Science (DIMO), University of Bari, Italy.
  • Elvira Grandone Atherosclerosis and Thrombosis Unit, IRCCS “Casa Sollievo della Sofferenza”, S. Giovanni Rotondo, Foggia, Italy.
  • Giuseppe Cardo Operative Unit of Urology “S. Giacomo” Hospital, Monopoli, Italy.
  • Donatella Colaizzo Atherosclerosis and Thrombosis Unit, IRCCS “Casa Sollievo della Sofferenza”, S. Giovanni Rotondo, Foggia, Italy.
  • Giuseppe Minoia Department of Animal Production, University of Bari, Italy.
  • Laura Castellana Department of Biomedical Sciences, University of Foggia, Italy.
  • Ugo Indraccolo Operative Unit of Gynaecology and Obstetrics of Civitanova Marche - Area Vasta 3, Marche, Italy.
  • Sonia Marrocchella Operative Unit of Obstetric and Gynecology, Department of Surgical Sciences, University of Foggia, Italy.
  • Pantaleo Greco Operative Unit of Obstetric and Gynecology, Department of Surgical Sciences, University of Foggia, Italy.
  • Raffaele Luigi Sciorsci Department of Animal Production, University of Bari, Italy.

Abstract

Background. Experimental evidence suggests a relationship between the vasodilatory effect of hCG and the NOS system in the testis. The influence of hCG administration on testicular vascular NOS gene expression has not been fully investigated. Objective: This study aimed to evaluate the presence of the nitric oxide syntheses gene in ram testicular arteries and the influence of hCG administration on its expression. Materials and methods: Both testicular arteries of sixteen rams were extracted before and after i.v. administration of 5000 IU of hCG or placebo. The expression of the iNOS gene was investigated by real time PCR. Data were analyzed by means of Wilcoxon and Mann-Whitney tests. A p value of < 0.05 was considered statistically significant. Results: PCR revealed the presence of iNOS mRNA in all basal samples but the expression of the iNOS gene was significantly reduced in all arteries obtained 24 h after the administration of either hCG or placebo. A significant reduction in the expression of iNOS gene was observed in the testicular arteries extracted after 24 h in both treated and placebo groups. On the other hand hCG stimulation did not significantly influence iNOS expression following its administration compared to a placebo. Conclusion: Ram testicular arteries express the iNOS gene but hCG stimulation did not significantly influence iNOS expression. A significant reduction in the expression of this gene was observed in the testicular arteries extracted after 24 h in both treated and placebo groups, suggesting that iNOS expression on the testicular artery could be influenced by the spermatic vessel ligation of the controlateral testis.

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Published
2014-09-30
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Section
Original Papers - Andrology
Keywords:
iNOS gene, Testicular artery, hCG, Ram, Intratesticular blood flow
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How to Cite
Matteo, M., Rizzo, A., Cicinelli, E., Grandone, E., Cardo, G., Colaizzo, D., Minoia, G., Castellana, L., Indraccolo, U., Marrocchella, S., Greco, P., & Sciorsci, R. L. (2014). Influence of hCG on inducible nitric oxide synthase gene expression in ram testicular arteries. Archivio Italiano Di Urologia E Andrologia, 86(3), 183-187. https://doi.org/10.4081/aiua.2014.3.183