First detection of the bloom forming Unruhdinium penardii (Dinophyceae) in a Mediterranean reservoir: insights on its ecology, morphology and genetics

Unruhdinium penardii in a Mediterranean reservoir

  • Cecilia Teodora Satta | ctsatta@uniss.it Department of Architecture, Design and Urban Planning, University of Sassari, Italy. https://orcid.org/0000-0003-0130-9432
  • Albert Reñé Institut de Ciències del Mar-CSIC, Dpt. Biologia Marina i Oceanografia, Barcelona, Spain.
  • Bachisio Mario Padedda Department of Architecture, design and urban planning, University of Sassari, Italy.
  • Silvia Pulina Department of Architecture, Design and Urban Planning, University of Sassari, Italy.
  • Giuseppina Grazia Lai Department of Architecture, Design and Urban Planning, University of Sassari, Italy.
  • Oriana Soru Ente Acque della Sardegna (ENAS), Cagliari, Italy.
  • Paola Buscarinu Ente Acque della Sardegna (ENAS), Cagliari, Italy.
  • Tomasa Virdis Ente Acque della Sardegna (ENAS), Cagliari, Italy.
  • Salvatore Marceddu Institut of Sciences of Food Production, National Research Council (CNR), Li Punti (SS), Italy.
  • Antonella Lugliè Department of Architecture, Design and Urban Planning, University of Sassari, Italy.

Abstract

The freshwater genus Unruhdinium includes dinoflagellates hosting a tertiary diatom endosymbiont. Some of the species belonging to this genus form high-biomass blooms. In this study, data on the ecology, morphology and molecular identity of Unruhdinium penardii were reported for the first time from a Mediterranean reservoir (Cedrino Lake, Sardinia, Italy). The ecology of the species and its bloom events were examined along a multiannual series of data (2010-2017). Cell morphology was investigated using field samples and six cultures established by cell isolation. A molecular identification of the six strains was performed. Wild and cultured cells shared the same morphology, showing a prominent apical pore complex and two/three more or less prominent hypothecal spines as distinctive characters in light microscopy. Molecularly, the six cultured strains corresponded to the same taxonomic entity with sequences only differing in a few polymorphic positions for the studied markers SSU rDNA, LSU rDNA, ITS and endosymbiont SSU rDNA. All markers showed 99.5%−100% similarity with the available U. penardii sequences. Seasonality of U. penardii revealed its preference for the colder semester (from December to June) with bloom events restricted to late winter/early spring months. Three blooms resulting in reddish water discolorations were observed along the study period (2011, 2012 and 2017). GLMs revealed a significant role of water depth, temperature, and reactive phosphorous in determining the highest cell densities (>5 x 104 cells L-1). The results obtained contribute to the increase of field ecology knowledge on this species, demonstrating it is well established in the Mediterranean area, and being able to produce recurrent high biomass blooms in the studied reservoir.

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Published
2021-01-07
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Keywords:
Peridiniopsis, cultures, temperature, cysts, endosymbiont
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How to Cite
Satta, C. T., Reñé, A., Padedda, B. M., Pulina, S., Lai, G. G., Soru, O., Buscarinu, P., Virdis, T., Marceddu, S., & Lugliè, A. (2021). First detection of the bloom forming <em>Unruhdinium penardii</em&gt; (Dinophyceae) in a Mediterranean reservoir: insights on its ecology, morphology and genetics: Unruhdinium penardii in a Mediterranean reservoir. Advances in Oceanography and Limnology, 11(2). https://doi.org/10.4081/aiol.2020.9500