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The partly varved and well-dated sediment record of Lake Tiefer See (NE Germany) allowed the high resolution paleolimnological reconstruction of the lake evolution during the whole Holocene. This paper presents results of subfossil Cladocera analysis. During the Holocene, the fauna of subfossil cladoceran was represented by 36 species belonging to 6 families. Cladocera were dominated by typical open-water species, belonging especially of the Bosminidae family. The sediment record of Lake Tiefer See exhibited distinct decadal- to centennial-scale alternations of well- and non-varved intervals, which were related to changes in the thermal circulation of the lake water column. In general, well varved sediments were deposited during periods of reduced lake circulation, and were characterised by maximum abundance of Cladocera, whereas non-varved sedimentation phases occurred during periods of increased lake circulation and showed a lower number of Cladocera specimens. The most suitable conditions for the development of cladoceran fauna occurred during the early Holocene and from ~ 2055 – 725 yr cal BP. On the basis of the increasing number of species associated with high lake productivity, eight stages of increasing trophy were inferred. The first two were attributed to climate warming, while the next six to human impact. Higher human driven trophic conditions of Lake Tiefer See occurred in the periods 5750-5500 and 4500-4100 yr cal BP, and four times from 1000 to50 yr cal BP. During the last 750 years and in the period from 6000 to 2500 yr cal BP, the species of Eubosmina produced extreme morphs. The cyclomorphosis of Eubosmina was likely connected to more pronounced changes in the lake environmental conditions.