https://doi.org/10.4081/ijfs.2024.12257
Molecular evidence of Toxoplasma gondii from the tissue and blood of naturally infected sheep
Submitted: 4 January 2024
Accepted: 29 February 2024
Published: 28 March 2024
Accepted: 29 February 2024
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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Toxoplasmosis is a cosmopolitan zoonotic infection that has significant effects on public health and causes economic losses in the livestock industry. The current study was designed to detect the Toxoplasma parasite in sheep blood samples and tissue samples of slaughtered sheep at the Sulaimani abattoir using molecular technique. A total of 300 peripheral sheep blood samples were randomly collected from 20 small ruminant flocks at 4 locations in the Sulaymaniyah province, northern Iraq. Also, 150 meat samples from thigh muscle, heart, and diaphragm were collected from slaughtered sheep. All collected blood samples were subjected to polymerase chain reaction (PCR) amplification to confirm Toxoplasma infection; in addition, meat samples were also analyzed for Toxoplasma by PCR following the digestion process. Of the 300 amplified blood samples, 94 were considered positive for Toxoplasma gondii, with a prevalence rate of 31.3%. The overall prevalence of Toxoplasma among meat samples was 34%. The diaphragm reported a higher infection rate (46%) than the heart (32%), while the femoral muscle reported an infection rate of 24%. Aged animals (older than 24 months) presented a higher infection rate (32.8%) than younger animals (28.9%). Contact with or consumption of uncooked meat from infected sheep increases the chance of parasite transmission to humans.
Abd El-Razik KA, Barakat AMA, Hussein HA, Younes AM, Elfadaly HA, Eldebaky HA, SolimanYA, 2018. Seroprevalence, isolation, molecular detection and genetic diversity of Toxoplasma gondii from small ruminants in Egypt. J Parasit Dis 42:527-36.
DOI: https://doi.org/10.1007/s12639-018-1029-4
Abdallah MC, Kamel M, Karima B, Samir A, Djamel K, Rachid K, Khatima A, 2019. Cross-sectional survey on Toxoplasma gondii Infection in cattle, sheep, and goats in Algeria: seroprevalence and risk factors. Vet Sci 6:63.
DOI: https://doi.org/10.3390/vetsci6030063
Al-Abodi HR, 2021. Diagnostic study to detect toxoplasmosis in some Iraqi sheep. J Phys Conf Ser 1879:022050.
DOI: https://doi.org/10.1088/1742-6596/1879/2/022050
Al-Shaibani KTM, Almaeahi AMY, Alsheabani MSA, 2019. Investigation of toxoplasmosis in sheep in Al-Diwaniya city by using modern technique. J Phys Conf Ser 1294:062062.
DOI: https://doi.org/10.1088/1742-6596/1294/6/062062
Amdouni Y, Rjeibi MR, Rouatbi M, Amairia S, Awadi S, Gharbi M, 2017. Molecular detection of Toxoplasma gondii infection in slaughtered ruminants (sheep, goats and cattle) in Northwest Tunisia. Meat Sci 133:180-4.
DOI: https://doi.org/10.1016/j.meatsci.2017.07.004
Amouei A, Sarvi S, Mizani A, Hashemi-Soteh MB, Salehi S, Javidnia J, Abdollah Hosseini SA, Amuei F, Alizadeh A, Shabanzade S, Gholami S, Daryani A, 2022. Genetic characterization of Toxoplasma gondii in meat-producing animals in Iran. Parasit Vectors 15:255.
DOI: https://doi.org/10.1186/s13071-022-05360-1
Anvari D, Saadati D, Nabavi R, Eskandani MA, 2018. Epidemiology and molecular prevalence of Toxoplasma gondii in cattle slaughtered in Zahedan and Zabol districts, south east of Iran. Iran J Parasitol 13:114-9.
Armand B, Solhjoo K, Shabani-Kordshooli M, Davami MH, Sadeghi M, 2016. Toxoplasma infection in sheep from south of Iran monitored by serological and molecular methods; risk assessment to meat consumers. Vet World 9:850-5.
DOI: https://doi.org/10.14202/vetworld.2016.850-855
Azizi H, Shiran B, Boroujeni AB, Jafari M, 2014. Molecular survey of Toxoplasma gondii in sheep, cattle and meat products in Chaharmahal va Bakhtiari province, southwest of Iran. Iran J Parasitol 9:429-34.
Bahreh M, Hajimohammadi B, Eslami G, 2021. Toxoplasma gondii in sheep and goats from central Iran. BMC Res Notes 14:46.
DOI: https://doi.org/10.1186/s13104-021-05465-3
Boyer KM, Holfels E, Roizen N, Swisher C, Mack D, Remington J, Withers S, Meier P, McLeod, R, 2005. Risk factors for Toxoplasma gondii infection in mothers of infants with congenital toxoplasmosis: implications for prenatal management and screening. Am J Obstet Gynecol 192:564-71.
DOI: https://doi.org/10.1016/j.ajog.2004.07.031
Cenci-Goga BT, Rossitto PV, Sachi P, McCrindle CME, Cullor JS, 2011. Toxoplasma in animals, food, and humans: an old parasite of new concern. Foodborne Pathog Dis 8:751-62.
DOI: https://doi.org/10.1089/fpd.2010.0795
Dubey JP, 2009. Toxoplasmosis in sheep - the last 20 years. Vet Parasitol 163:1-14.
DOI: https://doi.org/10.1016/j.vetpar.2009.02.026
Dubey JP, 2010. Toxoplasmosis of animals and humans. 2nd ed. CRC Press, Boca Raton, FL, USA.
Dubey JP, Beattie CP, 1988. Toxoplasmosis of animals and man. CRC Press, Boca Raton, FL, USA.
Ferreira Da Silva A, Brandão FZ, Carlos F, Oliveira R, Maria A, Ferreira R, 2013. Toxoplasma gondii in the sheep industry: a global overview and the situation in Brazil. R Bras Ci Vet 20:179-88.
DOI: https://doi.org/10.4322/rbcv.2014.012
Firouzeh N, Foroughiborj H, Kareshk AT, 2021. Genetic diversity of Toxoplasma gondii by serological and molecular analyzes in different sheep and goat tissues in northeastern Iran. Iran J Parasitol 18:217-28.
DOI: https://doi.org/10.21203/rs.3.rs-853244/v1
Gebremedhin EZ, Abdurahaman M, Haddish T, Tesserae, TS, 2014. Seroprevalence and risk factors of Toxoplasma gondii infection in sheep and goats slaughtered for human consumption in central Ethiopia. BMC Res Notes 7:696.
DOI: https://doi.org/10.1186/1756-0500-7-696
Gorji GRS, Rassouli M, Staji H, 2018. Prevalence of cerebral toxoplasmosis among slaughtered sheep in Semnan, Iran. Ann Parasitol 64:37-42.
Guo M, Dubey JP, Hill D, Buchanan RL, Ray Gamble H, Jones JL, Pradhan AK, 2015. Prevalence and risk factors for Toxoplasma gondii infection in meat animals and meat products destined for human consumption. J Food Prot 78:457-76.
DOI: https://doi.org/10.4315/0362-028X.JFP-14-328
Hamilton CM, Katzer F, Innes EA, Kelly PJ, 2014. Seroprevalence of Toxoplasma gondii in small ruminants from four Caribbean islands. Parasit Vectors 7:449.
DOI: https://doi.org/10.1186/1756-3305-7-449
Hill D, Dubey JP, 2002. Toxoplasma gondii: transmission, diagnosis and prevention. Clin Microbiol Infect 8:634-40.
DOI: https://doi.org/10.1046/j.1469-0691.2002.00485.x
Homan WL, Vercammen M, de Braekeleer J, Verschueren H, 2000. Identification of a 200- to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR p. Int J Parasitol 30:69-75.
DOI: https://doi.org/10.1016/S0020-7519(99)00170-8
Kareshk AT, Mahmoudvand H, Keyhani A, Tavakoli Oliaee R, Mohammadi MA, Babaei Z, Hajhosseini MA, Zia-Ali N, 2017. Molecular detection and genetic diversity of Toxoplasma gondii in different tissues of sheep and goat in eastern Iran. Trop Biomed 34:681-90.
Kijlstra A, Jongert E, 2008. Control of the risk of human toxoplasmosis transmitted by meat. Int J Parasitol 38:1359-70.
DOI: https://doi.org/10.1016/j.ijpara.2008.06.002
Klun I, Djurković-Djaković O, Katić-Radivojević S, Nikolić A, 2006. Cross-sectional survey on Toxoplasma gondii infection in cattle, sheep and pigs in Serbia: seroprevalence and risk factors. Vet Parasitol 135:121-31.
DOI: https://doi.org/10.1016/j.vetpar.2005.08.010
Kotula AW, Dubey JP, Sharar AK, Andrews CD, Shen SK, Lindsay DS, 1991. Effect of freezing on infectivity of Toxoplasma gondii tissue cysts in pork. J Food Prot 54:687-90.
DOI: https://doi.org/10.4315/0362-028X-54.9.687
Mikaeel FB, Al-Saeed ATM, 2020. Serological and molecular diagnosis of Toxoplasma gondii among ewes and horses in Duhok province. Iraq. Iraqi J Agric Sci 5:1212-9.
DOI: https://doi.org/10.36103/ijas.v51i4.1100
Motarjemi Y, Moy G, Todd ECD, 2014. Encyclopedia of food safety. 1st ed. Elsevier/Academic Press, Amsterdam, Netherlands.
Opsteegh M, Langelaar M, Sprong H, den Hartog L, de Craeye S, Bokken G, Ajzenberg D, Kijlstra, A, van der Giessen J, 2010. Direct detection and genotyping of Toxoplasma gondii in meat samples using magnetic capture and PCR. Int J Food Microbiol 139:193-201.
DOI: https://doi.org/10.1016/j.ijfoodmicro.2010.02.027
Rasti S, Marandi N, Abdoli A, Delavari M, Mousavi SGA, 2018. Serological and molecular detection of Toxoplasma gondii in sheep and goats in Kashan, central Iran. J Food Safety 38:e12425.
DOI: https://doi.org/10.1111/jfs.12425
Shapiro K, Bahia-Oliveira L, Dixon B, Dumètre A, A de Wit L, VanWormer E, Villena I, 2019. Environmental transmission of Toxoplasma gondii: oocysts in water, soil and food. Food Waterborne Parasitol 15:e00049.
DOI: https://doi.org/10.1016/j.fawpar.2019.e00049
Sroka J, Karamon J, Wójcik-Fatla A, Piotrowska W, Dutkiewicz J, Bilska-Zaja, E, Zają V, Kochanowski M, Dąbrowska J, Cencek T, 2020. Toxoplasma gondii infection in slaughtered pigs and cattle in Poland: seroprevalence, molecular detection and characterization of parasites in meat. Parasit Vectors 13:223.
DOI: https://doi.org/10.1186/s13071-020-04106-1
Swierzy IJ, Muhammad M, Kroll J, Abelmann A, Tenter AM, Lüder CGK, 2014. Toxoplasma gondii within skeletal muscle cells: a critical interplay for food-borne parasite transmission. Int J Parasitol 44:91-8.
DOI: https://doi.org/10.1016/j.ijpara.2013.10.001
Tenter AM, Heckeroth AR, Weiss LM, 2000. Toxoplasma gondii: from animals to humans. Int J Parasitol 30:1217-58.
DOI: https://doi.org/10.1016/S0020-7519(00)00124-7
Tilahun B, Tolossa YH, Tilahun G, Ashenafi H, Shimelis S, 2018. Seroprevalence and risk factors of Toxoplasma gondii infection among domestic ruminants in East Hararghe zone of Oromia region, Ethiopia. Vet Med Int 2018:4263470.
DOI: https://doi.org/10.1155/2018/4263470
Tonouhewa ABN, Akpo Y, Sessou P, Adoligbe C, Yessinou E, Hounmanou YG, Assogba MN, Youssao I, Farougou S, 2017. Toxoplasma gondii infection in meat animals from Africa: systematic review and meta-analysis of sero-epidemiological studies. Vet World 10:194-208.
DOI: https://doi.org/10.14202/vetworld.2017.194-208
Verma SK, Sweeny AR, Lovallo MJ, Calero-Bernal R, Kwok OC, Jiang T, Su C, Grigg ME, Dubey JP, 2017. Seroprevalence, isolation and co-infection of multiple Toxoplasma gondii strains in individual bobcats (Lynx rufus) from Mississippi, USA. Int J Parasitol 47:297-303.
DOI: https://doi.org/10.1016/j.ijpara.2016.12.007
Yang W, Lindquist HDA, Cama V, Schaefer FW III, Villegas E, Fayer R, Lewis EJ, Feng Y, Xiao L, 2009. Detection of Toxoplasma gondii oocysts in water sample concentrates by real-time PCR. Appl Environ Microbiol 75:3477-83.
DOI: https://doi.org/10.1128/AEM.00285-09
Yousefvand A, Mirhosseini SA, Ghorbani M, Mohammadzadeh T, Moghaddam MM, Mohammadyari S, 2021. Molecular and serological detection and of Toxoplasma gondii in small ruminants of southwest Iran and the potential risks for consumers. J Verbrauch Lebensm 16:117-27.
DOI: https://doi.org/10.1007/s00003-020-01306-w
How to Cite
1.
Abdullah SH. Molecular evidence of <i>Toxoplasma gondii</i> from the tissue and blood of naturally infected sheep. Ital J Food Safety [Internet]. 2024 Mar. 28 [cited 2024 Dec. 26];13(2). Available from: https://www.pagepressjournals.org/ijfs/article/view/12257
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