https://doi.org/10.4081/ijfs.2022.10350

Effect of gaseous ozone treatment on biofilm of dairy-isolated Pseudomonas spp. strains

Vol. 11 No. 2 (2022)
Submitted: 30 December 2021
Accepted: 24 February 2022
Published: 29 June 2022
Pseudomonas spp., Biofilm, Ozone, Dairy, Food safety
Abstract Views: 793
PDF: 318
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Authors

Felice Panebianco
felice.panebianco@unito.it
Department of Veterinary Sciences, University of Turin, Italy.
Selene Rubiola
Department of Veterinary Sciences, University of Turin, Italy.
Francesco Chiesa
Department of Veterinary Sciences, University of Turin, Italy.
Tiziana Civera
Department of Veterinary Sciences, University of Turin, Italy.
Pierluigi Aldo Di Ciccio
Department of Veterinary Sciences, University of Turin, Italy.

Microbial biofilms existing in food industries have been implicated as important contamination sources of spoilage and pathogenic microorganisms in the finished products. Among the innovative strategies proposed to contrast biofilms in food environments, ozone is recognised as an environmentally friendly technology but there are few studies about its effect against bacterial biofilms. The objective of this study was to evaluate the effect of gaseous ozone (50 ppm for 6 h) in inhibition and eradication of biofilm formed by twenty-one dairyisolated Pseudomonas spp. strains. Before ozone treatments, all isolates were screened for biofilm formation according to a previously described method. Strains were then divided in four groups: weak, weak/moderate, moderate/strong, and strong biofilm producers based on the biofilm biomass value of each isolate determined using the optical density (OD - 595 nm). Inhibition treatment was effective on the strain (C1) belonging to the weak producers’ group, on all strains classified as weak/moderate producers, on two strains (C8 and C12) belonging to the group of moderate/strong producers and on one strain (C13) classified as strong producer. Conversely, eradication treatments were ineffective on all strains tested, except for the strain C4 which reduced its biofilm-forming abilities after exposure to ozone gas. In conclusion, gaseous ozone may be used to enhance existing sanitation protocols in food processing environments, but its application alone not seems sufficient to contrast Pseudomonas spp. established biofilms.

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How to Cite

1.
Panebianco F, Rubiola S, Chiesa F, Civera T, Di Ciccio PA. Effect of gaseous ozone treatment on biofilm of dairy-isolated <em>Pseudomonas</em> spp. strains. Ital J Food Safety [Internet]. 2022 Jun. 29 [cited 2024 Nov. 21];11(2). Available from: https://www.pagepressjournals.org/ijfs/article/view/10350

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