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Occurrence of Aliarcobacter spp. in fresh and pre-cut vegetables of common use in San José, Costa Rica

Authors

María Laura Arias Echandi
maria.ariasechandi@ucr.ac.cr
https://orcid.org/0000-0002-8611-4433
Tropical Diseases Research Center, Faculty of Microbiology, University of Costa Rica, San José, Costa Rica.
Alejandra Huete Soto
Tropical Diseases Research Center, Faculty of Microbiology, University of Costa Rica, San José, Costa Rica.
Jose Manuel Castillo Blanco
Tropical Diseases Research Center, Faculty of Microbiology, University of Costa Rica, San José, Costa Rica.
Fernanda Fernández
https://orcid.org/0009-0004-9941-1690
Tropical Diseases Research Center, Faculty of Microbiology, University of Costa Rica, San José, Costa Rica.
Heriberto Fernandez Jaramillo
https://orcid.org/0000-0002-0009-7258
Institute of Clinical Microbiology, Universidad Austral de Chile, Valdivia, Chile.

Aliarcobacter is a Gram-negative rod that can cause disease in both animals and humans. Several studies have evidenced its presence in a wide variety of foods. Given that the number of foodborne illness outbreaks linked to the consumption of vegetables has increased worldwide and that there is a lack of information about the occurrence of Aliarcobacter spp. in these, the aim of this study was to evaluate its presence and the occurrence of virulence factors in both fresh and ready-to-eat vegetable samples. 180 vegetable samples from Costa Rica were analyzed for the presence of Aliarcobacter spp., including 90 pre-cut vegetable packages and 90 fresh vegetables. Two (2.2%) of the isolates from pre-cut vegetables and 19 (21.1%) of the isolates obtained from fresh vegetables were confirmed as Aliarcobacter spp. One of the isolates from the pre-cut vegetable samples was identified as A. butzleri. For the fresh vegetables, 11 isolates were identified as A. skirrowii, one as A. butzleri, and the 7 remaining isolates could not be identified at a species level. There is an 87.5% positivity for hecA and 93.8% for pldA, virulence genes in strains isolated from fresh produce, contrasting with an absolute absence from pre-cut vegetable-isolated strains. These results evidence the presence of Aliarcobacter on fresh and pre-cut vegetables from Costa Rica and the potential hazard it might represent for public health.

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Araya-Quesada Y, Jiménez-Robles A, Ivankovich-Guillén C, García-Barquero ME, 2014. Hábitos de consumo de embutidos en el cantón de San Carlos y el área metropolitana de Costa Rica. Tecnología en Marcha 27:113-24. [Article in Spanish].
Barboza K, Cubillo Z, Castro E, Redondo-Solano M, Fernández-Jaramillo H, Arias-Echandi ML, 2017. First isolation report of Arcobacter cryaerophilus from a human diarrhea sample in Costa Rica. Rev Inst Med Trop Sao Paulo 59:e72.
Calvo G, Arias ML, Fernández H, 2013. Arcobacter: un patógeno emergente de origen alimentario. ALN 63:164-72. [Article in Spanish].
Collado L, Figueras MJ, 2011. Taxonomy, epidemiology, and clinical relevance of the genus Arcobacter. Clin Microbiol Rev 24:174-92.
Collado L, Jara R, Vásquez N, Telsaint C, 2014. Antimicrobial resistance and virulence genes of Arcobacter isolates recovered from edible bivalve molluscs. Food Control 46:508-12.
D’sa EM, Harrison MA, 2005. Effect of pH, NaCl content, and temperature on growth and survival of Arcobacter spp. J Food Prot 68:18-25.
Douidah L, de Zutter L, Baré J, De Vos P, Vandamme P, Vandenberg O, Vander Abeele A, Houf K, 2012. Occurrence of putative virulence genes in Arcobacter species isolated from human and animal. J Clin Microbiol 50:735-41.
Ferreira S, Oleastro M, Domingues F, 2019. Current insights on Arcobacter butzleri in food chain. Curr Opin Food Sci 26:9-17.
Ferreira S, Queiroz JA, Oleastro M, Domingues FC, 2016. Insights in the pathogenesis and resistance of Arcobacter: a review. Crit Rev Microbiol 43:364-83.
Girbau C, Guerra C, Martínez-Malaxetxebarria I, Alonso R, Fernández-Astorga A, 2015. Prevalence of ten putative virulence genes in the emerging foodborne pathogen Arcobacter isolated from food products. Food Microbiol 52:146-9.
Harmon KM, Wesley IV, 1996. Identificatin of Arcobacter isolates by PCR. Lett Appl Microbiol 23:241-4.
Hausdorf L, Fröhling A, Schlüter O, Klocke M, 2011. Analysis of the bacterial community within carrot wash water. Can J Microbiol 57:447-52.
Hausdorf L, Neumann M, Bergmann I, Sobiella K, Mundt K, Fröhling A, Schlüter O, Klocke M, 2013. Occurrence and genetic diversity of Arcobacter spp. in a spinach-processing plant and evaluation of two Arcobacter-specific quantitative PCR assays. Syst Appl Microbiol 36:235-43.
Karadas G, Sharbati S, Hänel I, Messelhäußer U, Glocker E, Alter T, Gölz G, 2013. Presence of virulence genes, adhesion and invasion of Arcobacter butzleri. J Appl Microbiol 115:583-90.
Lake IR, Barker GC, 2018. Climate change, foodborne pathogens and illness in higher-income countries. Curr Envir Health Rpt 5:187-96.
MAG-MEIC, 2018. Decreto ejecutivo No. 41420-C2018. Publica Resolución N° 402-2018 (COMIECO-LXXXIII) de fecha 28/06/2018 y su Anexo:"Reglamento Técnico Centroamericano RTCA 67.04.50:17 Alimentos. Criterios Microbiológicos para la Inocuidad de los Alimentos". In: Official Journal, 238, 21/12/2018. [Decree in Spanish].
Molva C, Atabay HI, 2016. Prevalence and diversity of Arcobacter spp. in retail chicken meat in Turkey. Microbiol Res 7:6578.
Mottola A, Bonerba E, Bozzo G, Marchetti P, Celano GV, Colao V, Terio V, Tantillo G, Figueras MJ, Di Pinto A, 2016. Occurrence of emerging food-borne pathogenic Arcobacter spp. isolated from pre-cut (ready-to-eat) vegetables. Int J Food Microbiol 236:33-7.
Mottola A, Ciccarese G, Sinisi C, Savarino AE, Marchetti P, Terio V, Tantillo G, Barrasso R, Di Pinto A, 2021. Occurrence and characterization of Arcobacter spp. from ready-to-eat vegetables produced in Southern Italy. Ital J Food Saf 10:8585.
Neill SD, Ellis WA, O’Brien JJ, 1979. Designation of aerotolerant Campylobacter-like organisms from porcine and bovine abortions to the genus Campylobacter. Res Vet Sci 27:180-6.
Nieva-Echavarría B, Martinez-Malaxetxebarria I, Girbau C, Alonso R, Fernández-Astorga A, 2013. Prevalence and genetic diversity of Arcobacter in food products in the north of Spain. J Food Prot 76:1447-50.
Noto AM, Sciortino S, Cardamone C, Ciravolo C, Napoli C, Alio V, Arculeo P, Oliveri G, Costa A, 2018. Detection of Arcobacter spp. in food products collected from Sicilia region: a preliminary study. Ital J Food Saf 7:7171.
Perez-Cataluña A, Salas-Masso N, Diéguez AL, Balboa S, Lema A, Romalde JL, Figueras MJ, 2018. Revisiting the taxonomy of the genus Arcobacter: getting order from the chaos. Front Microbiol 9:2077
Pérez-Cataluña A, Salas-Massó N, Diéguez AL, Balboa S, Lema L, Romalde JL, Figueras MJ, 2019. Corrigendum (2): revisiting the taxonomy of the genus Arcobacter: getting order from the chaos. Front Microbiol 10:2253.
Ramees TP, Dhama K, Karthik K, Rathore RS, Kumar A, Saminathan M, Tiwari R, Malik YS, Singh RK, 2017. Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control - a comprehensive review. Vet Q 37:136-61.
Rivera Palomino GO, 2015. Determinación de la presencia de los genes putativos de virulencia de Arcobacter en diferentes medios de crecimiento, temperaturas y condiciones atmosféricas. Available from: https://bdigital.zamorano.edu/server/api/core/bitstreams/18ae2777-e9a8-4eff-aab6-ef143a17197a/content. [Material in Spanish].
Shah AH, Saleha AA, Zunita Z, Murugaiyah M. 2011. Arcobacter - an emerging threat to animals and animal origin food products? Trends Food Sci Technol 22:225-36.
Simaluiza RJ, Ambuludi DR, Fernández H, 2021. First case of diarrhea due to Aliarcobacter butzleri (formerly Arcobacter butzleri) in Ecuador. Infect Dis Now 51:564-6.
Vandamme P, Falsen E, Rossau R, Hoste B, Segers P, Tytgat R, De Ley J, 1991. Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int J Syst Bacteriol 41:88-103.
Vicente-Martins S, Oleastro M, Domingues FC, Ferreira S, 2018. Arcobacter spp. at retail food from Portugal: prevalence, genotyping and antibiotics resistance. Food Control 85:107-12.
Winters DK, Slavik MF, 2000. Multiplex PCR detection of Campylobacter jejuni and Arcobacter butzleri in food products. Molec Cell Probes 14:95-9.
Zacharow I, Bystroń J, Wałecka-Zacharska E, Podkowik M, Bania J, 2015. Genetic diversity and incidence of virulence-associated genes of Arcobacter butzleri and Arcobacter cryaerophilus isolates from pork, beef, and chicken meat in Poland. Biomed Res Int 2015:956507.

How to Cite

1.
Arias Echandi ML, Huete Soto A, Castillo Blanco JM, Fernández F, Fernandez Jaramillo H. Occurrence of <i>Aliarcobacter</i> spp. in fresh and pre-cut vegetables of common use in San José, Costa Rica. Ital J Food Safety [Internet]. 2023 Oct. 2 [cited 2025 Apr. 3];12(4). Available from: https://www.pagepressjournals.org/ijfs/article/view/10344
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