https://doi.org/10.4081/ijfs.2022.10289

Evaluation of the microbiological and chemical aspects of autochthonous wild snails in Sardinia

Vol. 11 No. 2 (2022)
Submitted: 17 November 2021
Accepted: 9 March 2022
Published: 29 June 2022
Snails, Listeria monocytogenes, Salmonella spp, Heavy metals, Food safety
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Authors

Valentina Coroneo
coroneo@unica.it
Department of Medical Sciences and Public Health, University of Cagliari, Italy.
Luisa Marras
Department of Medical Sciences and Public Health, University of Cagliari, Italy.
Valerio Giaccone
Department of Animal Medicine, Production and Health, University of Padova, Italy.
Daniele Conficoni
Department of Animal Medicine, Production and Health, University of Padova, Italy.
Silvana Anna Stefania Brignardello
Istituto Zooprofilattico Sperimentale della Sardegna, Food and Water Microbiology Laboratory, Elmas (CA), Italy.
Elisa Bissacco
EPTA Nord SRL Food Analysis and Consulting - Conselve (PD), Italy.
Claudio Trapella
Department of Chemistry and Pharmaceutical Sciences, University of Ferrara, Italy.
Andrea Alogna
Department of Chemistry and Pharmaceutical Sciences, University of Ferrara, Italy.
Valentina Gentili
Department of Chemistry and Pharmaceutical Sciences, University of Ferrara, Italy.
Antonio Parisi
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Italy.
Federica Loi
Osservatorio Epidemiologico Veterinario Regionale, IZS-Sardegna, Cagliari, Italy.
Stefano Cappai
Osservatorio Epidemiologico Veterinario Regionale, IZS-Sardegna, Cagliari, Italy.
Margherita Pisanu
Istituto Zooprofilattico Sperimentale della Sardegna, Sassari - Laboratorio Microbiologia – Terreni Colturali e Prodotti Vegetali, Sassari, Italy.
Maria Paola Cogoni
Istituto Zooprofilattico Sperimentale della Sardegna, Food and Water Microbiology Laboratory, Elmas (CA), Italy.

This study was conducted to acquire knowledge on the epidemiology and ecology of some zoonotic agents in snails. Chemical and microbiological analysis was carried out on 46 samples of snails belonging to the species of Helix aspersa and Helix (Eobania) vermiculata. The association between heavy metals and wild snails, a native consumer product in the Region of Sardinia, was determined. The molecular characterisation of Listeria monocytogenes virulence genes has shown a genetic profile that deserves more attention for the improvement of surveillance and risk prevention. Specimens of H. vermiculata showed higher concentrations of cadmium (M=0.80±0.56 mg/kg) than H. aspersa (M=0.61±0.17 mg /kg). A further objective was to determine whether the samples showed significant differences from the point of view of secretion characterisation, in terms of protein content, and to identify species-specific correlations and possible relationships with the environment. The presence of Salmonella enterica sub.sp houtenae (6,14: z4, z23) and Salmonella enterica subsp diarizonae (47: k: e, n, z15) (1 sample), Listeria monoSnails,cytogenes (2 samples) with Molecular characterization of virulence genes together with the measurement of heavy metals in samples of wild snails has shown a health and hygiene profile that would deserve greater attention for the improvement of the surveillance and prevention of microbiological and chemical risk in such products which currently show a tendency towards increase in consumption.

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Citations

Crossref
Scopus
Google Scholar
Europe PMC
Barker GM, 2011. The biology of terrestrial molluscs. cap 2-8, cap 14. CABI publishing doi 10.1079/9780851993188.0000
Boshoff M, Jordaens K, Backeljau T, Lettens S, Tack F, Vandecasteele B, De Jonge M, Bervoets L, 2013. Organ- and species-specific accumulation of metals in two land snail species (Gastropoda, Pulmonata). Sci Total Environ 449:470-81. DOI: https://doi.org/10.1016/j.scitotenv.2013.02.003
Cicero A, Giangrosso G, Cammilleri G, Macaluso A, Currò V, Galuppo L, Vargetto D, Vicari D, Ferrantelli V, 2015. Microbiological and Chemical Analysis of Land Snails Commercialised in Sicily. Ital J Food Safety 4:4196. DOI: https://doi.org/10.4081/ijfs.2015.4196
Commission Regulation (EC) No 1881/2006, 2006. Maximum levels of certain contaminants in foodstuffs, amendments by Reg. EC 1126/2007 and Reg. EC 629/2008, 2006.
Commission Regulation (EU) No 488/2014, 2014. Amending Regulation (EC) No 1881/2006 as regards maximum cadmium levels in foodstuffs.
Commission Regulation (EC) No 1441/2007, 2007. Amending Regulation (EC) No 2073/20.
Corda A, Mara L, Virgilio S, Pisanu M, Chessa G, Parisi A, Cogoni MP, 2014. Microbiological and chemical evaluation of Helix spp. snails from local and non-EU markets, utilised as food in Sardinia. Ital J Food Safety 3:1732. DOI: https://doi.org/10.4081/ijfs.2014.1732
Coroneo V, Carraro V, Aissani N, Sanna A, Ruggeri A, Succa S, Meloni B, Pinna A, Sanna C, 2016. Detection of Virulence Genes and Growth Potential in Listeria monocytogenes Strains Isolated from Ricotta Salata Cheese. J Food Sci 81:M114–20. DOI: https://doi.org/10.1111/1750-3841.13173
EFSA, 2013. The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks in 201. EFSA J 11:3129. DOI: https://doi.org/10.2903/j.efsa.2013.3129
Huang F, Peng L, Zhang J, Lin W, Chen S, 2018. Cadmium bioaccumulation and antioxidant enzyme activity in hepatopancreas, kidney, and stomach of invasive apple snail Pomacea canaliculata. Environ Sci Pollut Res Int 25:18682-92. DOI: https://doi.org/10.1007/s11356-018-2092-1
Jesse R, Véla E, Pfenninger M, 2011. Phylogeography of a land snail suggests trans-Mediterranean neolithic transport. PloS One 6:e20734. DOI: https://doi.org/10.1371/journal.pone.0020734
Marras L, Carraro V, Sanna C, Sanna A, Ingianni A, Coroneo V, 2019. Growth of Listeria monocytogenes in ready to eat salads at different storage temperatures and valuation of virulence genes expression. Ann Ig Med Prev Comunita 31:374-84.
Massadeh AM, Alomary AA, Mir S, Momani FA, Haddad HI, Hadad YA, 2016. Analysis of Zn, Cd, As, Cu, Pb, and Fe in snails as bioindicators and soil samples near traffic road by ICP-OES. Sci Pollut Res Int 23:13424-31. DOI: https://doi.org/10.1007/s11356-016-6499-2
Parisi A, Latorre L, Normanno G, Miccolupo A, Fraccalvieri R, Lorusso V, Santagada G, 2010. Amplified Fragment Length Polymorphism and Multi-Locus Sequence Typing for high-resolution genotyping of Listeria monocytogenes from foods and the environment. Food Microbiol 27:101-8. DOI: https://doi.org/10.1016/j.fm.2009.09.001
Radzki RP, Bieńko M, Polak P, Szkucik K, Ziomek M, Ostapiuk M, Bieniaś J, 2018. Is the consumption of snail meat actually healthy? An analysis of the osteotropic influence of snail meat as a sole source of protein in growing rats. J Anim Physiol Anim Nutr 102:e885-91. DOI: https://doi.org/10.1111/jpn.12851
Scheifler R, Gomot-de Vaufleury A, Badot PM, 2002. Transfer of cadmium from plant leaves and vegetable flour to the snail Helix aspersa: bioaccumulation and effects. Ecotoxicol Environ Safety 53:148-53. DOI: https://doi.org/10.1006/eesa.2002.2216

How to Cite

1.
Coroneo V, Marras L, Giaccone V, Conficoni D, Brignardello SAS, Bissacco E, Trapella C, Alogna A, Gentili V, Parisi A, Loi F, Cappai S, Pisanu M, Cogoni MP. Evaluation of the microbiological and chemical aspects of autochthonous wild snails in Sardinia. Ital J Food Safety [Internet]. 2022 Jun. 29 [cited 2024 Nov. 21];11(2). Available from: https://www.pagepressjournals.org/ijfs/article/view/10289

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