Isolation and identification of Helicobacter pylori from raw chicken meat in Dhamar Governorate, Yemen

Submitted: 15 October 2021
Accepted: 15 February 2022
Published: 21 June 2022
Abstract Views: 939
PDF: 321
HTML: 63
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Although Helicobacter pylori (H. pylori) is one of the most common bacterial pathogens of human, its natural reservoirs are still unclear. There is an increasing number of reports that document the occurrence of H. pylori in various foods. This study aimed at isolation of H. pylori from chicken meat sampled. Two hundred and sixty samples were collected randomly from slaughterhouses and markets in Dhamar Governorate, Yemen. Samples were enriched in Brain-Heart Infusion broth in microaerophilic conditions before inoculating the Camp-Blood agar and EYE agar plates. Results showed that 13.8% of samples were contaminated evidenced by H. pylori growth via traditional culture method on agar media. No significant differences between sample types (thighs and breast muscles) (p=0.353) or the sampling source (p=0.816) were observed. Autumn season was associated with increased occurrence of H. pylori. The source of H. pylori in food is still not identified. Proper cooking and good sanitation practices are highly recommended to avoid the infection. Further studies addressing the potential sources of H. pylori are highly suggested.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Al-Mashhadany D, Mayass SM, 2017. Incidence of Helicobacter pylori in food and water in Dhamar governorate/Yemen. Int J Curr Res 9:45320–6.
Almashhadany D, Mayass SM, 2018. Prevalence of Helicobacter pylori in Human in Dhamar Governorate / Yemen. J Med Pharm Sci 2:1–18.
Al-mashhadany DA, 2018. Application of Stool Antigen Test for Monitoring Helicobacter pylori among Human in Erbil Governorate, Kurdistan Region, Iraq Int J Pharm Pharm Sci 10:49–3.
Al-Mashhadany DA, 2020. Epidemiology of Helicobacter pylori among Human at Erbil Governorate/Kurdistan Region / Iraq. World J Pharm Pharm Sci 9: 435–47.
Al-Shamahy HA, 2005. Seroprevalence of Helicobacter pylori among children in Sana’a, Yemen. Ann Saudi Med 25:299–03. DOI: https://doi.org/10.5144/0256-4947.2005.299
Almashhadany DA, 2021a. Impact of heat treatment on the antimicrobial residues in raw goat’s milk. Iraqi J Vet Sci 35:549–3. DOI: https://doi.org/10.33899/ijvs.2020.127137.1469
Almashhadany DA, 2021b. Diagnosis of brucellosis in sheep and goats raw milk by fast and reliable techniques. Iraqi J Vet Sci 35:663–8. DOI: https://doi.org/10.33899/ijvs.2021.127697.1523
Al-Mashhadany DA, Ismael LQ, Zaki AM, 2018. Seroprevalence of Helicobacter pylori among human in Erbil governorate, Kurdistan region, Iraq Res J Life Sci Bioinform Pharm Chem Sci 4:268–80. DOI: https://doi.org/10.22159/ijpps.2018v10i11.28872
Alsulaimany FAS, Awan ZA, Almohamady AM, Koumu MI, Yaghmoor BE, Elhady SS, Elfaky MA, 2020. Prevalence of Helicobacter pylori Infection and Diagnostic Methods in the Middle East and North Africa Region. Medicina (B. Aires). 56:169. DOI: https://doi.org/10.3390/medicina56040169
Atapoor S, Dehkordi FS, Rahimi E, 2014. Detection of Helicobacter pylori in Various Types of Vegetables and Salads. Jundishapur J Microbiol 7:10013. DOI: https://doi.org/10.5812/jjm.10013
Bahumid NM, Al-Kazimi G, Taresh AS, Abdullah HS, Khamis I, 2009. An epidemiological view of Helicobacter pylori infection among patients who underwent upper gastrointestinal tract endoscopy in Aden. Univ Aden J Nat Appl Sci 13:159–64.
Begue RE, Gonzales JL, Correa-Gracian H, Tang S, 1998. Dietary risk factors associated with the transmission of Helicobacter pylori in Lima, Peru. Am J Trop Med Hyg 59:637–40. DOI: https://doi.org/10.4269/ajtmh.1998.59.637
Coldham T, Rose K, O'rourke J, Neilan BA, Dalton H, Lee A, Mitchell H, 2011. Detection, isolation, and characterization of Helicobacter species from the gastrointestinal tract of the brushtail possum. Appl environ microbiol 77:1581-7. DOI: https://doi.org/10.1128/AEM.01960-10
Denic M, Touati E, Reuse H De, 2020. Pathogenesis of Helicobacter pylori infection. Helicobacter 25:e12736. DOI: 10.1111/HEL.12736. DOI: https://doi.org/10.1111/hel.12736
Dore MP, Sepulveda AR, El-Zimaity H, Yamaoka Y, Osato MS, Mototsugu K, Nieddu AM, Realdi G, Graham DY, 2001. Isolation of Helicobacter pylori from sheep—implications for transmission to humans. Am J Gastroenterol 96:1396–401. DOI: https://doi.org/10.1111/j.1572-0241.2001.03772.x
Duynhoven YTHP van & Jonge R de, 2001. Transmission of Helicobacter pylori: a role for food? Bull World Health Organ 79: 455–60.
Gunaid AA, Hassan NA, Murray-Lyon I, 2003. Prevalence and risk factors for Helicobacter pylori infection among dyspeptic patients. Saudi Med J 24:512–7.
Hamada M, Elbehiry A, Marzouk E, Moussa IM, Hessain AM, Alhaji JH, Heme HA, Zahran R, & Abdeen E, 2018. Helicobacter pylori in a poultry slaughterhouse: Prevalence, genotyping and antibiotic resistance pattern. Saudi J Biol Sci 25:1072–8. DOI: https://doi.org/10.1016/j.sjbs.2018.02.002
Hemmatinezhad B, Momtaz H, Rahimi E, 2016. VacA, cagA, iceA and oipA genotypes status and antimicrobial resistance properties of Helicobacter pylori isolated from various types of ready to eat foods. Ann Clin Microbiol Antimicrob 15:1–9. DOI: https://doi.org/10.1186/s12941-015-0115-z
Herrera AG, 2004. Helicobacter pylori and Food Products: A Public Health Problem. In: Spencer JFT, de Spencer RAL, eds. Public Health Microbiology: Methods and Protocols. Humana Press Inc, Totowa, NJ. pp. 297–301. DOI: https://doi.org/10.1385/1-59259-766-1:297
Kazemeini H, Rahimi E, Kianpour F, 2014. Prevalence of Helicobacter pylori in buffalo milk in Iran. Iran J Public Health 43:174.
Keenan JI, Salm N, Hampton MB, Wallace AJ, 2010. Individual and combined effects of foods on Helicobacter pylori growth. Phyther Res 24: 1229–33. DOI: https://doi.org/10.1002/ptr.3167
Lawson AJ, 2015. Helicobacter. In: Jorgensen JH, Pfaller MA, eds. Manual of Clinical Microbiology 11th ed., John Wiley & Sons, Ltd., New York, NY, pp 1013–1027. DOI: https://doi.org/10.1128/9781555817381.ch57
Meng X, Zhang H, Law J, Tsang R, Tsang T, 2008. Detection of Helicobacter pylori from food sources by a novel multiplex PCR assay. J. Food Saf. 28: 609–619. DOI: https://doi.org/10.1111/j.1745-4565.2008.00135.x
Momtaz H, Dabiri H, Souod N, Gholami M, 2014. Study of Helicobacter pylori genotype status in cows, sheep, goats and human beings. BMC Gastroenterol 14:1–7. DOI: https://doi.org/10.1186/1471-230X-14-61
Mousavi S, Dehkordi FS, Rahimi E, 2015. Virulence factors and antibiotic resistance of Helicobacter pylori isolated from raw milk and unpasteurized dairy products in Iran. J Venom Anim Toxins Incl Trop Dis 20:1–7. DOI: https://doi.org/10.1186/1678-9199-20-51
Quaglia NC, Dambrosio A, 2018. Helicobacter pylori: A foodborne pathogen? World J Gastroenterol 24:3472. DOI: https://doi.org/10.3748/wjg.v24.i31.3472
Quaglia NC, Dambrosio A, Normanno G, Parisi A, Firinu A, Lorusso V, Celano G V, 2007. Survival of Helicobacter pylori in artificially contaminated ultrahigh temperature and pasteurized milk. Food Microbiol 24:296–300. DOI: https://doi.org/10.1016/j.fm.2006.04.008
Rahimi E, Kheirabadi EK, 2012. Detection of Helicobacter pylori in Bovine, Buffalo, Camel, Ovine, and Caprine Milk in Iran. Foodborne Pathog Dis 9:453–6. DOI: https://doi.org/10.1089/fpd.2011.1060
Saeidi E, Sheikhshahrokh A, 2016. VacA genotype status of Helicobacter pylori isolated from foods with animal origin. Biomed Res Int 2016:1–6. DOI: https://doi.org/10.1155/2016/8701067
Sjomina O, Pavlova J, Niv Y, Leja M, 2018. Epidemiology of Helicobacter pylori infection. Helicobacter 23:e12514. DOI: https://doi.org/10.1111/hel.12514
Talaei R, Souod N, Momtaz H, Dabiri H, 2015. Milk of livestock as a possible transmission route of Helicobacter pylori infection. Gastroenterol Hepatol from Bed to Bench 8: S30.
Talimkhani A, Mashak Z, 2017. Prevalence and Genotyping of Helicobacter pylori Isolated From Meat, Milk and Vegetable in Iran. Jundishapur J Microbiol 2017:10.. DOI: https://doi.org/10.5812/jjm.14240
Vale FF, Vítor JMB, 2010. Transmission pathway of Helicobacter pylori: Does food play a role in rural and urban areas? Int J Food Microbiol 138:1–12. DOI: https://doi.org/10.1016/j.ijfoodmicro.2010.01.016
Yahaghi E, Khamesipour F, Mashayekhi F, Dehkordi FS, Sakhaei MH, Masoudimanesh M, Khameneie MK, 2014. Helicobacter pylori in vegetables and salads: Genotyping and antimicrobial resistance properties. Biomed Res Int 2014. DOI: https://doi.org/10.1155/2014/757941

How to Cite

1.
Almashhadany DA, Mayas SM, Ali NL. Isolation and identification of <em>Helicobacter pylori</em> from raw chicken meat in Dhamar Governorate, Yemen. Ital J Food Safety [Internet]. 2022 Jun. 21 [cited 2024 Dec. 22];11(2). Available from: https://www.pagepressjournals.org/ijfs/article/view/10220

Similar Articles

You may also start an advanced similarity search for this article.