https://doi.org/10.4081/hls.2024.12456

The amount of DNA and RNA in primary cancer cells and their metastases

Early Access
Submitted: 5 March 2024
Accepted: 10 May 2024
Published: 22 August 2024
Carcinogenesis, advanced molecular genetic analysis, microspectrophotometry, nucleic acids, oncodiagnosis, non-cell autonomous effectors
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Authors

Oleksandr Herasymenko
herasymenkool@ukr.net
Department of Pathological Anatomy, Forensic Medicine, Histology, Donetsk National Medical University, Kropyvnytskyi, Ukraine.
Kostyantyn Gerasymenko
Department of Pathological Anatomy, Forensic Medicine, Histology, Donetsk National Medical University, Kropyvnytskyi, Ukraine.
Olha Herasymenko
Feofaniya Clinical Hospital, Kyiv, Ukraine.

Considering the significant impact of oncopathology on global morbidity and mortality, as well as changes in its prevalence and typology, research into the molecular-genetic mechanisms of oncogenesis and metastasis, particularly the role of nucleic acids, becomes crucial. The aim of this study is to determine the content of DNA and RNA in cells of primary cancer and its metastases through microspectrophotometric analysis of malignant cells collected from 30 patients with primary and metastatic oncological processes. The following methods were employed: microspectrophotometric analysis using the automated complex “Protva-PM11-DVK-3m,” staining of histological specimens with halo cyanin-chrome alum, the discrete statistics method, frequency and cluster analysis, and the bibliographic method. Changes in the concentration of nucleic acids in the cells of primary and metastatic tumors were identified during the study. The changes did not always correlate with each other, indicating morphofunctional polymorphism in tumor cell populations. A trend towards a decrease in nuclear RNA concentration and an increase in cytoplasmic RNA concentration in metastases were observed, suggesting alterations in metabolic and synthetic processes in cells and a decrease in their differentiation degree.

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How to Cite

Herasymenko, O., Gerasymenko, K., & Herasymenko, O. (2024). The amount of DNA and RNA in primary cancer cells and their metastases. Healthcare in Low-Resource Settings. https://doi.org/10.4081/hls.2024.12456
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