https://doi.org/10.4081/hls.2024.11988
Anti-biofilm properties of clover honey against Candida albicans
Submitted: 27 October 2023
Accepted: 21 December 2023
Published: 16 February 2024
Accepted: 21 December 2023
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All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Authors
Candida albicans grows rapidly when the microflora becomes imbalanced due to a variety of factors. Its ability to infect a host is aided by its virulence factors, such as biofilm. This study aimed to evaluate the activity of clover honey in inhibiting and degrading the biofilm formation of C. albicans in vitro. This study used a true experimental design with an in vitro post-test-only control group design approach. The microtiter plate assay was used to grow planktonic cells and biofilm. This method was carried out to obtain the Optical Density (OD) value for each test, measured by a Microplate Reader. Cell viability was measured using the MTS Assay kit, the biofilm matrix was measured using the Crystal Violet Assay, and the morphology of C. albicans biofilms was observed by scanning electron microscopy (SEM). Probit and One-way ANOVA tests were applied to determine the MIC50 of both planktonic and biofilm, as well as statistical analysis. The results showed that clover honey exerted inhibitory activity against C. albicans planktonic cells at a MIC50value of 31.60% w/v. At the highest concentration, clover honey exhibited antibiofilm activity by lowering the extracellular matrix and viability of C. albicans cells by 64.59% and 72.09%, respectively. Based on SEM observation, clover honey changed the cell morphology of C. albicans and reduced the thickness of the biofilm. Overall, our findings concluded that clover honey exhibited antifungal properties against C. albicans by inhibiting biofilm formation and degrading mature biofilm.
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How to Cite
Masfufatun, M., Setiawan, B., Purbowati, R., Tjandra, L., Indahsari, N. K., Ratnasari, D. T., Narottama, H., & Arimbi, M. R. (2024). Anti-biofilm properties of clover honey against <i>Candida albicans</i>. Healthcare in Low-Resource Settings, 12(1). https://doi.org/10.4081/hls.2024.11988
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