https://doi.org/10.4081/ejtm.2022.10673

The effect of Lactobacillus casei and Bacillus coagulans probiotics on liver damage induced by silver nanoparticles and expression of Bax, Bcl2 and Caspase 3 genes in male rats

Vol. 33 No. 1 (2023)
Submitted: 13 June 2022
Accepted: 21 June 2022
Published: 13 September 2022
Silver nanoparticles, probiotics, liver enzymes, Bax, Bcl2, Caspase-3, rat
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Authors

Reyhaneh Oliaei
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran, Islamic Republic of.
Zahra Keshtmand
zkeshtmand2001@gmail.com
Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran, Islamic Republic of.
Ronak Shabani
Department of Anatomical Sciences, School of Medicine, Iran University of Medical Sciences, Tehran, Iran, Islamic Republic of.

In this study, we aimed to evaluate the effect of Bacillus coagulans and Lactobacillus casei probiotics on liver damage induced by silver nanoparticles and expression of Bax, Bcl2 and Caspase 3 genes in rats. 32 adult male Wistar rats were divided into four healthy groups (control), the group receiving silver nanoparticles treated with L. casei, the group receiving silver nanoparticles treated with B. coagulans and the group receiving only silver nanoparticles. The effect of nanoparticles was induced by intraperitoneal injection of silver nanoparticles prepared from nettle at a dose of 50 mg/kg and entered the liver tissue through the bloodstream. Two days after injection, probiotic treatment with 109 CFU was performed by gavage for 30 days. One day after the last gavage, rat liver tissue weight was assessed. Also, the total amount of RNA was extracted from treated, and healthy tissues, as well as induced silver nanoparticles tissues, then evaluated by Real Time PCR. Data were evaluated using one-way Anova, Tukey test. Based on the biochemical results of this study, exposure of rats to different concentrations of silver nanoparticles compared with the control group caused a significant increase in the serum levels of alanine transaminase (ALT) and aspartate transaminase (AST), alkaline phosphatase (ALP), especially at high concentrations. Evaluation of damage and histopathological lesions showed that silver nanoparticles in different concentrations caused different damage to liver tissue, so that, necrosis, inflammatory cell infiltration and vascular degeneration were observed at different concentrations by silver nanoparticles. In the present study, the effects of L. casei cell extract on increasing the expression of Bax proapoptotic gene and decreasing Bcl2 gene expression in cancer cells and inducing programmed cell death were shown. In this study, the expression of Bax, Bcl-2 and Caspase-3 genes in the group receiving silver nanoparticles and in the groups treated with probiotics showed significant changes compared to the control group. It can be concluded that the function of silver nanoparticles and the effects of relative improvement of probiotics are from the internal route of apoptosis and factors such as dose, nanoparticle size and nanoparticle coating have an important role in the toxicity of silver nanoparticles, thus the destructive effects on liver tissue could be increased by increasing the concentration of silver nanoparticles.

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Citations

Crossref
Scopus
Google Scholar
Europe PMC
Mitra V, Metcalf J. Metabolic functions of the liver. Anaes Inten. Care Med. 2012:13(2):54-55. DOI: https://doi.org/10.1016/j.mpaic.2011.11.006
Tuñón MJ, Alvarez M, Culebras JM, González-Gallego J. An overview of animal models for investigating the pathogenesis and therapeutic strategies in acute hepatic failure. World J Gastroenterol. 2009 Jul 7;15(25):3086-98. DOI: https://doi.org/10.3748/wjg.15.3086
Oravecz K, Kalka D, Jeney F, Cantz M, Zs-Nagy I. Hydroxyl free radicals induce cell differentiation in SK-N-MC neuroblastoma cells. Tissue Cell. 2002 Feb;34(1):33-8. DOI: https://doi.org/10.1054/tice.2001.0221
Völker C, Boedicker C, Daubenthaler J, Oetken M, Oehlmann J. Comparative toxicity assessment of nanosilver on three Daphnia species in acute, chronic and multi-generation experiments. PLoS One. 2013 Oct 7;8(10):e75026. DOI: https://doi.org/10.1371/journal.pone.0075026
Hendi A. Silver nanoparticles mediate differential responses in some of liver and kidney functions during skin wound healing. J King Saud Uni. 2011; 23(1): 47–52. DOI: https://doi.org/10.1016/j.jksus.2010.06.006
Kim JS, Sung JH, Ji JH, Song KS, Lee JH, Kang CS, Yu IJ. In vivo Genotoxicity of Silver Nanoparticles after 90-day Silver Nanoparticle Inhalation Exposure. Saf Health Work. 2011 Mar;2(1):34-8. Epub 2011 Mar 31. DOI: https://doi.org/10.5491/SHAW.2011.2.1.34
Lee JM, Lee MA, Do HN, Song YI, Bae RJ, Lee HY, Park SH, Kang JS, Kang JK. Historical control data from 13-week repeated toxicity studies in Crj:CD (SD) rats. Lab Anim Res. 2012 Jun;28(2):115-21. Epub 2012 Jun 26. DOI: https://doi.org/10.5625/lar.2012.28.2.115
Honarvar F, Vaezi G, Nourani M, Kamrani A, Sadeghnezhad E. Oxidant/Antioxidant index evaluation in the rat embryo induced by Nano-silver particle. NCMBJ. 2016; 6 (23) :53-60
Ritze Y, Bárdos G, Claus A, Ehrmann V, Bergheim I, Schwiertz A, Bischoff SC. Lactobacillus rhamnosus GG protects against non-alcoholic fatty liver disease in mice. PLoS One. 2014 Jan 27;9(1):e80169. DOI: https://doi.org/10.1371/journal.pone.0080169
Moradi HR, Erfani Majd N, Esmaeilzadeh S, Fatemi Tabatabaei SR. The histological and histometrical effects of Urtica dioica extract on rat's prostate hyperplasia. Vet Res Forum. 2015 Winter;6(1):23-9. Epub 2015 Mar 15.
Tang J, Xi T. [Status of biological evaluation on silver nanoparticles]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2008 Aug;25(4):958-61. Chinese.
Naghsh N, Noori A, Aqababa H, amirkhani S. Effect of Nanosilver Particles on Alanin Amino Transferase (ALT) Activity and White Blood Cells (WBC) Level in Male Wistar Rats, In Vivo Condition. Zahedan J Res Med Sci. 2012;14(7):e93312.
Zamani N, Naghsh N, Fathpour H. Comparing poisonous effects of thioacetamide and silver nanoparticles on enzymic changes and liver tissue in mice. Zahedan J Res Med Sci (ZJRMS) 2014; 16(2): 54-57.
Johnston HJ, Hutchison G, Christensen FM, Peters S, Hankin S, Stone V. A review of the in vivo and in vitro toxicity of silver and gold particulates: particle attributes and biological mechanisms responsible for the observed toxicity. Crit Rev Toxicol. 2010 Apr;40(4):328-46. DOI: https://doi.org/10.3109/10408440903453074
Park EJ, Bae E, Yi J, Kim Y, Choi K, Lee SH, Yoon J, Lee BC, Park K. Repeated-dose toxicity and inflammatory responses in mice by oral administration of silver nanoparticles. Environ Toxicol Pharmacol. 2010 Sep;30(2):162-8. Epub 2010 May 19. DOI: https://doi.org/10.1016/j.etap.2010.05.004
Hirayama K, Raftar J. The role of probiotic bacteria in cancer prevention. Microbe Infect.,2000:2(6);687-68. DOI: https://doi.org/10.1016/S1286-4579(00)00357-9
Biffi A, Coradini D, Larsen R, Riva L, Di Fronzo G. Antiproliferative effect of fermented milk on the growth of a human breast cancer cell line. Nutr Cancer. 1997;28(1):93-9. DOI: https://doi.org/10.1080/01635589709514558
Sekine K, Toida T, Saito M, Kuboyama M, Kawashima T, Hashimoto Y. A new morphologically characterized cell wall preparation (whole peptidoglycan) from Bifidobacterium infantis with a higher efficacy on the regression of an established tumor in mice. Cancer Res. 1985 Mar;45(3):1300-7.
Zimmermann KC, Bonzon C, Green DR. The machinery of programmed cell death. Pharmacol Ther. 2001 Oct;92(1):57-70. PMID: 11750036. DOI: https://doi.org/10.1016/S0163-7258(01)00159-0
Yu WJ, Son JM, Lee J, Kim SH, Lee IC, Baek HS, Shin IS, Moon C, Kim SH, Kim JC. Effects of silver nanoparticles on pregnant dams and embryo-fetal development in rats. Nanotoxicology. 2014 Aug;8 Suppl 1:85-91. Epub 2013 Nov 22. DOI: https://doi.org/10.3109/17435390.2013.857734
Carraro U, Franceschi C. Apoptosis of skeletal and cardiac muscles and physical exercise.Aging (Milano). 1997 Feb-Apr;9(1-2):19-34. DOI: https://doi.org/10.1007/BF03340125
Sandri M, Carraro U. Apoptosis of skeletal muscles during development and disease. Int J Biochem Cell Biol. 1999 Dec;31(12):1373-90. Review. DOI: https://doi.org/10.1016/S1357-2725(99)00063-1
Ghavami S, Hashemi M, Ande SR, Yeganeh B, Xiao W, Eshraghi M, Bus CJ, Kadkhoda K, Wiechec E, Halayko AJ, Los M. Apoptosis and cancer: mutations within caspase genes. J Med Genet. 2009 Aug;46(8):497-510. Epub 2009 Jun 7. PMID: 19505876. DOI: https://doi.org/10.1136/jmg.2009.066944
Adrain C, Martin SJ. The mitochondrial apoptosome: a killer unleashed by the cytochrome seas. Trends Biochem Sci. 2001 Jun;26(6):390-7. doi: 10.1016/s0968-0004(01)01844-8. DOI: https://doi.org/10.1016/S0968-0004(01)01844-8

How to Cite

Oliaei, R., Keshtmand, Z., & Shabani, R. (2022). The effect of <em>Lactobacillus casei</em> and <em>Bacillus coagulans</em> probiotics on liver damage induced by silver nanoparticles and expression of Bax, Bcl2 and Caspase 3 genes in male rats. European Journal of Translational Myology, 33(1). https://doi.org/10.4081/ejtm.2022.10673
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