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Evaluation of human bone marrow mesenchymal stem cells in the treatment of non obstructive azoospermia

Authors

Mohamed A. Alhefnawy
dr.mohamedalhefnawy@gmail.com
https://orcid.org/0000-0002-0370-4607
Urology, Benha University, Benha, Egypt.
Gamal Elmorsy
Clinical Pathology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
Sayed Bakry
https://orcid.org/0000-0003-4198-0175
Genetic Engineering, Faculty of Science for Boys in Cairo, Al-Azhar University, Cairo, Egypt.
Hesham El-amrosy
Clinical Pathology, Egypt Ministry of Health and Population, Cairo, Egypt.
Ibrahim Mearaj
Dermatology and Andrology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt.
Ebrahim A. Sabra
https://orcid.org/0000-0002-7239-8033
Animal cell and tissue culture, Genetic engineering and Biotechnology Institute, Sadat University, Sadat City, Egypt.
Osama M. Badr
Animal cell and tissue culture, Genetic engineering and Biotechnology Institute, Sadat University, Sadat City, Egypt.
Dalia Ibraheem
Department of Tissue engineering, Faculty of Science, Al-Azhar University, Cairo, Egypt.
Taymour Khalifa
Dermatology and Andrology , Al-Azhar University Faculty of Medicine, Cairo, Egypt.

Background: Non-obstructive azoospermia (NOA) represents an infertility problem that is usually difficult to treat. Such patients usually have testicular biopsy of germ cell aplasia or spermatogenic arrest. In recent decades, mesenchymal stem cells (MSCs) had been studied thoroughly and proved safe and effective regarding their capability for trans-differentiation into different cell types. The aim of this study was to evaluate the effect of MSCs local intratesticular injection in induction of spermatogenesis.
Patients and method: The current study included 87 infertile non-obstructive azoospermic patients. Clinical assessment and repeated semen analysis with centrifugation were done to confirm azoospermia. Karyotyping and AZF study were done. Some of the patients had previous testicular biopsy proving a lack of sperm in the testes. Single intratesticular injection of purified MSCs suspension was done.
Results: 20.7% of patients showed sperm in their semen after variable period of time. Hormonal profile among treated patients showed significant improvement regardless success of treatment. Also most of the treated patients appreciated the improvement of their sexual function and libido.
Conclusions: Bone marrow derived MSCs could be a new hope and therapeutic modality for treatment of refractory cases of NOA.

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Ahmad Majzoub, Marina C. Viana, Arnold P. P. Achermann, Isadora T. Ferreira, Rita J. Laursen, Peter Humaidan, Sandro C. Esteves (2024)
Non-Obstructive Azoospermia and Intracytoplasmic Sperm Injection: Unveiling the Chances of Success and Possible Consequences for Offspring. Journal of Clinical Medicine, 13(16), 4939.
10.3390/jcm13164939
Azizoglu M. (2025)
Regenerative medicine in surgery: stem cells and exosome applications. Cirugia y Cirujanos, 93(1), 86-104.
10.24875/CIRU.24000620
Caroppo E, Colpi GM. Update on the Management of Non-Obstructive Azoospermia: Current Evidence and Unmet Needs. J Clin Med. 2021;11:62. DOI: https://doi.org/10.3390/jcm11010062
Wosnitzer M, Goldstein M and Hardy MP. Review of Azoospermia. Spermatogenesis. 2014;4:e28218. DOI: https://doi.org/10.4161/spmg.28218
Boivin J, Bunting L, Collins JA, Nygren KG. International estimates of infertility prevalence and treatment-seeking: potential need and demand for infertility medical care. Hum Reprod. 2007;22:1506-12. DOI: https://doi.org/10.1093/humrep/dem046
Oud MS, Ramos L, O'Bryan MK, et al. Validation and application of a novel integrated genetic screening method to a cohort of 1,112 men with idiopathic azoospermia or severe oligozoospermia. Hum Mutat. 2017;38:1592-605. DOI: https://doi.org/10.1002/humu.23312
Kanatsu-Shinohara M, Toyokuni S, Morimoto T, et al. Functional assessment of self-renewal activity of male germline stem cells following cytotoxic damage and serial transplantation. Biol Reprod. 2003;68:1801-7. DOI: https://doi.org/10.1095/biolreprod.102.012575
Ferlin A, Raicu F, Gatta V, et al. Male infertility: role of genetic background. Reprod Biomed Online. 2007;14:734-45. DOI: https://doi.org/10.1016/S1472-6483(10)60677-3
Caroppo E, Colpi EM, Gazzano G, et al. The seminiferous tubule caliber pattern as evaluated at high magnification during microdissection testicular sperm extraction predicts sperm retrieval in patients with non-obstructive azoospermia. Andrology. 2019;7:8-14. DOI: https://doi.org/10.1111/andr.12548
Valli H, Gassei K, Orwig KE. Stem cell therapies for male infertility: where are we now and where are we going? In: DT Carrell, PN Schlegel, C Racowsky, L Gianaroli, Editors. Biennial Review of Infertility. Springer, Cham: 2015; p. 17-39. DOI: https://doi.org/10.1007/978-3-319-17849-3_3
Kim HJ, Park JS. Usage of Human Mesenchymal Stem Cells in Cell-based Therapy: Advantages and Disadvantages. Dev Reprod. 2017; 21:1-10. DOI: https://doi.org/10.12717/DR.2017.21.1.001
Chikhovskaya JV, Jonker MJ, Meissner A, et al. Human testisderived embryonic stem cell-like cells are not pluripotent, but possess potential of mesenchymal progenitors. Hum Reprod. 2012; 27:210-21. DOI: https://doi.org/10.1093/humrep/der383
Wei Y, Fang J, Cai S, et al. Primordial germ cell-like cells derived from canine adipose mesenchymal stem cells. Cell Prolif. 2016;49:503-11. DOI: https://doi.org/10.1111/cpr.12271
Wang Y, Yi H, Song Y. The safety of MSC therapy over the past 15 years: a meta-analysis. Stem Cell Res Ther. 2021; 12:545. DOI: https://doi.org/10.1186/s13287-021-02609-x
Ghasemzadeh-Hasankolaei M, Batavani R, Eslaminejad MB, Sayahpour F. Transplantation of Autologous Bone Marrow Mesenchymal Stem Cells into the Testes of Infertile Male Rats and New Germ Cell Formation. Int J Stem Cells. 2016;9:250-63. DOI: https://doi.org/10.15283/ijsc16010
Wang F, Liu C, Zhang S, et al. Transplantation of goat bone marrow mesenchymal stem cells (MScs) help restore spermatogenesis in endogenous germ cells-depleted mouse models. J Integr Agric 2013;12:483-94. DOI: https://doi.org/10.1016/S2095-3119(13)60249-X
Caroppo E, Colpi GM. Hormonal treatment of men with nonobstructive azoospermia: What does the evidence suggest? J Clin Med 2021;10:387. DOI: https://doi.org/10.3390/jcm10030387
Chen C. From mesenchymal stem cell therapy to discovery of drug therapy for systemic sclerosis. University of Southern California, ProQuest Dissertations Publishing 2014;3628-36.
Liu F, Qiu H, Xue M, et al. MSC-secreted TGF-b regulates lipopolysaccharide-stimulated macrophage M2-like polarization via the Akt/FoxO1 pathway. Stem Cell Res Ther. 2019;10:345. DOI: https://doi.org/10.1186/s13287-019-1447-y
Zhang S, Teo KYW, Chuah SJ, et al. MSC exosomes alleviate temporomandibular joint osteoarthritis by attenuating inflammation and restoring matrix homeostasis. Biomaterials. 2019;200:35-47. DOI: https://doi.org/10.1016/j.biomaterials.2019.02.006
Cakici C, Buyrukcu B, Duruksu G, et al. Recovery of fertility in azoospermia rats after injection of adipose-tissue-derived mesenchymal stem cells: the sperm generation. Biomed Res Int. 2013;013:529589. DOI: https://doi.org/10.1155/2013/529589
Lue Y, Erkkila K, Liu PY, et al. Fate of bone marrow stem cells transplanted into the testis: potential implication for men with testicular failure. Am J Pathol. 2007;170:899-908. DOI: https://doi.org/10.2353/ajpath.2007.060543
Yang S, Bo J, Hu H, et al. Derivation of male germ cells from induced pluripotent stem cells in vitro and in reconstituted seminiferous tubules. Cell Prolif. 2012;45:91-100. DOI: https://doi.org/10.1111/j.1365-2184.2012.00811.x
Zhang D, Liu X, Peng J, et al. Potential spermatogenesis recovery with bone marrow mesenchymal stem cells in an azoospermic rat model. Int J Mol Sci. 2014;15:13151-65. DOI: https://doi.org/10.3390/ijms150813151
Pittenger MF, Discher DE, Péault BM, et al. Mesenchymal stem cell perspective: cell biology to clinical progress. NPJ Regen Med. 2019;4:22. DOI: https://doi.org/10.1038/s41536-019-0083-6
Cassim MI, Mohamed T. Novel therapy for the treatment of male factor infertility due to non-obstructive azoospermia: a case report. Cresc J Med Biol Sci. 2019;6:129-131.
Baghaei K, Hashemi SM, Tokhanbigli S, et al. Isolation, differentiation, and characterization of mesenchymal stem cells from human bone marrow. Gastroenterol Hepatol Bed Bench. 2017;10:208-213.
Neri S. Genetic Stability of Mesenchymal Stromal Cells for Regenerative Medicine Applications: A Fundamental Biosafety Aspect. Int J Mol Sci. 2019;20:2406. DOI: https://doi.org/10.3390/ijms20102406
Fedder J, Marcussen N, Fedder MDK, Engvad B. Testicular Damage following Testicular Sperm Retrieval: A Ram Model Study. Biomed Res Int. 2017; 2017:2472805. DOI: https://doi.org/10.1155/2017/2472805
Eliveld J, van Wely M, Meißner A, et al. The risk of TESEinduced hypogonadism: a systematic review and meta-analysis. Hum Reprod Update. 2018;24:442-54. DOI: https://doi.org/10.1093/humupd/dmy015

How to Cite

Alhefnawy, M. A., Elmorsy, G., Bakry, S., El-amrosy, H., Mearaj, I., Sabra, E. A., Badr, O. M., Ibraheem, D., & Khalifa, T. (2024). Evaluation of human bone marrow mesenchymal stem cells in the treatment of non obstructive azoospermia. Archivio Italiano Di Urologia E Andrologia, 96(1). https://doi.org/10.4081/aiua.2024.12285
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