https://doi.org/10.4081/mm.2011.2352

Typing of Enterococcus spp. strains isolated from patients with infective endocarditis by an automated repetitive-sequence-based PCR system

Vol. 26 No. 3 (2011)
Submitted: 13 February 2014
Accepted: 13 February 2014
Published: 30 September 2011
rep-PCR, Enterococcus spp, Antimicrobial susceptibility testing, Infective endocarditis
Abstract Views: 1419
PDF: 1051
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Authors

Giuseppe Ivan Russello
farina.claudio@sancarlo.mi.it
Claudio Farina
Annibale Raglio
Veronica Ravasio
on behalf of the Italian Infective Endocarditis Study Group (SEI)
The use of rep-PCR was studied to characterize 32 isolates of Enterococcus spp. from Italian patients with infective endocarditis and determine specific banding patterns associated with antimicrobial resistance phenotypes. The results obtained with rep-PCR were analyzed with respect to the antimicrobial profile by a MIC evaluation (E-test) of all strains tested. The automated rep-PCR technique resulted an easy method for the characterization of Enterococcus spp. It confirmed the eterogenicity of enterococcal strains involved in infective endocarditis. Furthermore results of antimicrobial susceptibility revealed that rep-PCR can not be used as diagnostic tool to characterize and identify difference between resistant and susceptible Enterococcus spp. strains.

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How to Cite

Russello, G. I., Farina, C., Raglio, A., Ravasio, V., & Endocarditis Study Group (SEI), on behalf of the I. I. (2011). Typing of Enterococcus spp. strains isolated from patients with infective endocarditis by an automated repetitive-sequence-based PCR system. Microbiologia Medica, 26(3). https://doi.org/10.4081/mm.2011.2352

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