Application of an innovative technique for histological and histochemical study of different vegetal tissues

Submitted: October 20, 2014
Accepted: October 20, 2014
Published: January 30, 2009
Abstract Views: 673
PDF: 656
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A new original "cryostabilization" technique (Dore, 1992; Dore et al, 2004) was applied to different animal tissues and organs. In this study, we apply this technique also to vegetal tissues (ripening and ripe fleshy fruits, buds and embryos). The proposed method preserves good tissue morphology and intracellular substances, and avoids loss of epicuticular waxes; moreover it maintains in situ some enzymatic activities. Being some samples very difficult to handle the original protocol had to be modified. The correct orientation in embedding of samples is an important requirement which is not easy to achieve when deadling with small fragments of tissues.

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Dore, B., Ponso, A., & Vallania, R. (2009). Application of an innovative technique for histological and histochemical study of different vegetal tissues. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 82(1). https://doi.org/10.4081/jbr.2009.4789