Alterations in the expression of genes for tubulin and stress in two Arabidopsis auxin mutants during simulated microgravity

Submitted: July 4, 2014
Accepted: July 4, 2014
Published: January 30, 2011
Abstract Views: 755
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The effect of microgravity on the expression of genes for tubulin, stress and aging in Arabidopsis seedlings from wild-type, and the auxinic mutants aux 1 and eir/pin2 is reported. Simulated microgravity was produced through the Random Positioning Machine (RPM). The results showed that the tubulin genes TUA4, TUA6 and TUB2, in plants subjected to μg, showed a peak of expression at 3h in the wild-type, whereas in the mutants it was delayed to 6h. The microgravity treatment tested on the expression of stress genes FeSOD1 and CAT3 showed ony a significant increase of senescence SAG12 showed increased expression in the wild-type starting at 3h, whereas in the mutant a significant increase was seen only at 24h. From the collected data we conclude that auxin transport and action should be involved in tubulin synthesis, stress, and the senescence process.

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Tassone, P., Fortunati, A., Saba, A., Pani, G., Meloni, M., Pippia, P., & Migliaccio, F. (2011). Alterations in the expression of genes for tubulin and stress in two Arabidopsis auxin mutants during simulated microgravity. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 84(1). https://doi.org/10.4081/jbr.2011.4495