https://doi.org/10.4081/jbr.2014.2138

Magnesium intracellular content and distribution map in drug-resistant and -sensitive whole cells

Vol. 87 No. 1 (2014): Proceedings of the 85th SIBS Congress
Submitted: November 21, 2013
Accepted: November 21, 2013
Published: January 10, 2014
magnesium, XRFM, AFM, drug-resistance
Abstract Views: 1234
PDF: 1045
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Emil Malucelli
emil.malucelli@unibo.it
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Stefano Lagomarsino
Department of Physics, Institute of Chemical and Physical processess, La Sapienza University, Roma, Italy.
Lucia Merolle
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Chiara Marraccini
Department of Life Sciences, University of Modena and Reggio Emilia, Italy.
Azzurra Sargenti
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Concettina Cappadone
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Giovanna Farruggia
Department of Pharmacy and Biotechnologies, University of Bologna, Italy.
Alessia Cedola
Institute of Photonics and Nanotechnology – CNR, Roma, Italy.
Michela Fratini
Institute of Photonics and Nanotechnology – CNR, Roma, Italy.
Andrea Notargiacomo
Institute of Photonics and Nanotechnology – CNR, Roma, Italy.
Stefano Iotti
National Institute of Biostructures and Biosystems, Roma, Italy.
Magnesium (Mg) plays crucial structural and regulatory roles within cells. Despite the extensive amount of data about the biochemistry of Mg, a complete picture of its regulation and cellular homeostasis is lacking. Thanks to recent improvements in third generation synchrotron X-ray sources, X-ray fluorescence microscopy (XRFM) is becoming a highly sensitive method for mapping elemental distributions in cells. XRFM maps the element content but not the concentration, which is a relevant variable in a biological context. We tackled this issue by combining XRFM with atomic force microscopy that was used to obtain morphological information of the sample. The aim of the present study was to compare the content and the distribution of Mg in drug-resistant and -sensitive tumor cell lines. Our data has shown a massive increase of Mg in LoVo drug-resistant cells. Moreover, the map of intracellular Mg showed marked differences in the pattern distribution between sensitive and resistant cells.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Crossref
Scopus
Google Scholar
Europe PMC

How to Cite

Malucelli, E., Lagomarsino, S., Merolle, L., Marraccini, C., Sargenti, A., Cappadone, C., Farruggia, G., Cedola, A., Fratini, M., Notargiacomo, A., & Iotti, S. (2014). Magnesium intracellular content and distribution map in drug-resistant and -sensitive whole cells. Journal of Biological Research - Bollettino Della Società Italiana Di Biologia Sperimentale, 87(1). https://doi.org/10.4081/jbr.2014.2138

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.