Development of an innovative microcantilever-based biosensor for 17β-estradiol detection in bovine muscles: preliminary results

Submitted: 15 January 2013
Accepted: 21 April 2013
Published: 10 June 2013
Abstract Views: 1236
PDF: 1495
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17β-estradiol is the most powerful substance with estrogenic effect, commonly used as illegal growth promoter in livestock production. To avoid health risks for consumers, sensitive, reliable and low-cost methods for quantification of extremely low concentrations of such carcinogenic residues in food are needed. Antibody-immobilised microcantilever resonators were proposed as innovative biosensors able to quantify an adsorbed target mass thanks to a shift in resonance frequency. Furthermore, the quantification of masses on the order of few picograms has recently shown to be successfully achievable with very high precision. In this study, we analysed the performance of our microcantilever sensors using extracted samples of bovine muscle from experimental animals, containing variable concentrations of 17β-estradiol (HPLC-MS/MS tested). Preliminary data showed that treated animals are correctly revealed, exhibiting large negative frequency shifts. More experiments, though, are needed to obtain a correct quantification of 17β-estradiol concentration.

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Pitardi D, Pezzolato M, Richelmi G, Gili M, Di Corcia D, Ciprianetti N, Ferrante I, Santoro K, Ricciardi C, Bozzetta E. Development of an innovative microcantilever-based biosensor for 17β-estradiol detection in bovine muscles: preliminary results. Ital J Food Safety [Internet]. 2013 Jun. 10 [cited 2024 Nov. 13];2(1):e12. Available from: https://www.pagepressjournals.org/ijfs/article/view/ijfs.2013.e12