Quantitative determination of quinolones residues in milk by HPLC-FLD

Submitted: 4 January 2013
Accepted: 4 January 2013
Published: 31 October 2012
Abstract Views: 1426
PDF: 1413
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Veterinary drugs have become an integral part of the livestock production and play an important role in maintenance of animal welfare. The use of veterinary medicines may be cause of the presence of drug residues in animal food products if appropriate withdrawal periods are not respected or if contaminated feeds are used. This work presents the development of an HPLC-FLD method for the quantitative de-tection of eight quinolones – norfloxacin, ciprofloxacin, danofloxacin, enrofloxacin, difloxacin, oxolinic acid, nalidixic acid, flumequine– in bovine milk. After deproteination and extraction with a metaphos-phoric acid 1% w/v / methanol / acetonitrile (60/20/20 v/v/v) solution, the sample is partially evaporated and cleaned up on a reversed phase SPE cartridge.The extract is analyzed using an high performance liquid chromatograph with fluorescence detector. Mean recovery ranged between 65% - 88%. All the an-alytes can be identified and quantified in the concentration range 15 - 60 μg/Kg for danofloxacin and 25 - 150 μg/Kg for the other quinolones.

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1.
Gili M, Marchis D, Stella P, Olivo F, Ostorero F, Franzoni M, Abete MC. Quantitative determination of quinolones residues in milk by HPLC-FLD. Ital J Food Safety [Internet]. 2012 Oct. 31 [cited 2024 Nov. 22];1(5):35-8. Available from: https://www.pagepressjournals.org/ijfs/article/view/ijfs.2012.5.35