https://doi.org/10.4081/ijfs.2017.6154

Development of a method to extract and amplify the complete mitogenome of some Sparidae species

Vol 6 no. 2 (2017)
Submitted: 13 July 2016
Accepted: 13 February 2017
Published: 22 June 2017
Sparidae, Mitogenome, Method development, Food control
Abstract Views: 1177
PDF: 557
HTML: 250
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

Celestina Mascolo
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples; Biology and Evolution of Marine Organisms, Research Centre Anton Dohrn Zoological Station, Naples, Italy.
Marina Ceruso
marina.ceruso@gmail.com
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Paolo Sordino
Biology and Evolution of Marine Organisms, Research Centre Anton Dohrn Zoological Station, Naples, Italy.
Giuseppe Palma
Assoittica Italia, Rome, Italy.
Aniello Anastasio
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Tiziana Pepe
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Naples, Italy.
Previous studies showed that fish mitochondrial DNA (mtDNA) is set up by a closed circular molecule of 16-17 kilobases (kb), comprising 2 ribosomal RNA genes (rRNA), 22 transfer RNA genes (tRNA), 13 protein-coding genes and 2 non-coding regions. The analysis of single mtDNA genes, such as Cytb, COI, 16S and 12S, or short segment of them, has been widely used against species substitution in both fresh and processed fish products. The analysis of the complete mitochondrial genome of fishery products allows to better study and characterise fish species. The aim of this research was to extract and amplify the complete mtDNA of some fish species of commercial interest belonging to the Sparidae family. The studied species were Dentex dentex, Dentex gibbosus, Dentex nufar, Pagellus acarne and Pagellus erythrinus. The entire mitogenome was obtained by gene amplification using long polymerase chain reactions. The analysis of the complete mitochondrial sequences will allow to gain further insights on these species and to find polymorphic sites that assess the degree of genetic variability of the species belonging to the family Sparidae.

Dimensions

Altmetric

PlumX Metrics

Downloads

Download data is not yet available.

Citations

Crossref
Scopus
Google Scholar
Europe PMC

How to Cite

1.
Mascolo C, Ceruso M, Sordino P, Palma G, Anastasio A, Pepe T. Development of a method to extract and amplify the complete mitogenome of some Sparidae species. Ital J Food Safety [Internet]. 2017 Jun. 22 [cited 2024 Nov. 12];6(2). Available from: https://www.pagepressjournals.org/ijfs/article/view/6154

PAGEPress has chosen to apply the Creative Commons Attribution NonCommercial 4.0 International License (CC BY-NC 4.0) to all manuscripts to be published.