https://doi.org/10.4081/ijfs.2009.1153

EVALUATION OF LISTERIA MONOCYTOGENES CONTAMINATION IN RETAIL SMOKED SALMON

AIVI - September 2009 - Issue 5
Submitted: 13 February 2013
Accepted: 13 February 2013
Published: 13 September 2009
Smoked salmon, L. monocytogenes, ISO 11290-1, ISO 11290-2, 16S rDNA, PCR
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Authors

P. Serratore
lucia.zoppi@pagepress.org
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale - Università di Bologna, .
A Piano
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale - Università di Bologna, .
J. Galletti
Corso di Laurea di Acquacoltura ed Igiene delle Produzioni Ittiche - Università di Bologna, .
M Trentini
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale - Università di Bologna, .
E. Zavatta
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale - Università di Bologna, .
S. Piraccini
Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale - Università di Bologna, .
The aim of this study was to assess the contamination levels of L. monocytogenes in retail smoked salmon of different geographical origin, comparing the performance of two isolation methods, ISO 11290-1 (qualitative) and ISO 11290-2 (quantitative), and also by molecular methods (16S rDNA sequencing, PCR with specific primers). Among 35 samples (7 batches, 5 samples per batch), only 1 sample from Denmark resulted positive for L. monocytogenes. The strains isolated on ALOA and Palcam Agar by the qualitative methods, were confirmed to the specie level (iap+, InlA+, InlB+), belonging to the serotype 1/2c - 3c (D2+), and positive for the virulence genes (InlC+ e InlJ+).

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How to Cite

1.
Serratore P, Piano A, Galletti J, Trentini M, Zavatta E, Piraccini S. EVALUATION OF LISTERIA MONOCYTOGENES CONTAMINATION IN RETAIL SMOKED SALMON. Ital J Food Safety [Internet]. 2009 Sep. 13 [cited 2024 Nov. 12];1(5):91-2. Available from: https://www.pagepressjournals.org/ijfs/article/view/ijfs.2009.5.91

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