Cover Image

Peripheral endocannabinoids regulate skeletal muscle development and maintenance

Dongjiao Zhao, Amber Pond, Bruce Watkins, Dave Gerrard, Yefei Wen, Shihuan Kuang, Kevin Hannon
  • Dongjiao Zhao
    Department of Basic Medical Sciences, Purdue University, West Lafayette, IN, United States
  • Amber Pond
    Department of Basic Medical Sciences, Purdue University, West Lafayette, IN, United States
  • Bruce Watkins
    Department of Food Sciences, Purdue University, West Lafayette, IN, United States
  • Dave Gerrard
    Department of Animal Sciences, Virginia Tech University, Blacksburg, VA, United States
  • Yefei Wen
    Department of Animal Sciences, Purdue University, West Lafayette, IN, United States
  • Shihuan Kuang
    Department of Animal Sciences, Purdue University, West Lafayette, IN, United States
  • Kevin Hannon
    Department of Basic Medical Sciences, Purdue University, West Lafayette, IN, United States | Hannonk@purdue.edu

Abstract

As a principal tissue responsible for insulin-mediated glucose uptake, skeletal muscle is important for whole-body health. The role of peripheral endocannabinoids as regulators of skeletal muscle metabolism has recently gained a lot of interest, as endocannabinoid system disorders could cause peripheral insulin resistance. We investigated the role of the peripheral endocannabinoid system in skeletal muscle development and maintenance. Cultures of C2C12 cells, primary satellite cells and mouse skeletal muscle single fibers were used as model systems for our studies. We found an increase in cannabinoid receptor type 1 (CB1) mRNA and endocannabinoid synthetic enzyme mRNA skeletal muscle cells during differentiation. We also found that activation of CB1 inhibited myoblast differentiation, expanded the number of satellite cells, and stimulated the fast-muscle oxidative phenotype. Our findings contribute to understanding of the role of the endocannabinoid system in skeletal muscle metabolism and muscle oxygen consumption, and also help to explain the effects of the peripheral endocannabinoid system on whole-body energy balance.

Keywords

Endocannabinoids, skeletal muscle, development, differentiation, metabolism

Full Text:

PDF
Submitted: 2013-07-03 14:16:03
Published: 2010-12-03 00:00:00
Search for citations in Google Scholar
Related articles: Google Scholar
Abstract views:
472

Views:
PDF
239

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


Copyright (c) 2010 Dongjiao Zhao, Amber Pond, Bruce Watkins, Dave Gerrard, Yefei Wen, Shihuan Kuang, Kevin Hannon

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 
© PAGEPress 2008-2018     -     PAGEPress is a registered trademark property of PAGEPress srl, Italy.     -     VAT: IT02125780185     •     Privacy