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A mini-review of TAT-MyoD fused proteins: state of the art and problems to solve

Marco Patruno, Luca Melotti, Chiara Gomiero, Roberta Sacchetto, Ohad Topel, Tiziana Martinello
  • Marco Patruno
    Department of Comparative Biomedicine and Food Science, University of Padova, Italy | marco.pat@unipd.it
  • Luca Melotti
    Department of Comparative Biomedicine and Food Science, University of Padova, Italy
  • Chiara Gomiero
    Department of Comparative Biomedicine and Food Science, University of Padova, Italy
  • Roberta Sacchetto
    Department of Comparative Biomedicine and Food Science, University of Padova, Italy
  • Ohad Topel
    VTH - Koret School of Veterinary Medicine, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Israel
  • Tiziana Martinello
    Department of Comparative Biomedicine and Food Science, University of Padova, Italy

Abstract

The transcriptional activator TAT is a small peptide essential for viral replication and possesses the property of entering the cells from the extracellular milieu, acting as a membrane shuttle. In order to safely differentiate cells an innovative methodology, based on the fusion of transcription factors and the TAT sequence, is discussed in this short review. In several studies, it has been demonstrated that TAT protein can be observed in the cell nucleus after few hours from the inoculation although its way of action is not fully understood. However, further studies will be necessary to develop this methodology for clinical purposes.

Keywords

Tat-MyoD, C2C12, PB-MSCs, coculture, myogenic induction

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Submitted: 2016-05-29 18:17:35
Published: 2017-12-05 15:56:11
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Copyright (c) 2017 Marco Patruno

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