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Functional electrical stimulation as a safe and effective treatment for equine epaxial muscle spasms: Clinical evaluations and histochemical morphometry of mitochondria in muscle biopsies

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Functional Electrical Stimulation (FES) has been used extensively over several decades to reverse muscle atrophy during rehabilitation for spinal cord injury patients. The benefits of the technology are being expanded into other areas, and FES has been recently utilized for injury rehabilitation and performance enhancement in horses. Six retired horses (age from 10 to 17 yrs) that had been previously used mainly for dressage riding were selected for this study. Clinical evaluation found epaxial muscle spasms in all horses with minimal to no pelvic extension when manually palpated. FES treatments were performed on the sacral/lumbar region 3 times per week for a period of 8 weeks, obtaining a total of 22 treatments per horse. The Modified Ashworth Scale for grading muscle spasms found a one grade improvement after approximately four FES treatments, indicating improved functional movement of the sacral/lumbar region, supporting the evidence by clinical palpations that a reduction in epaxial muscle spasms occurred. Skeletal muscle biopsies Pre and Post FES treatments were obtained from the longissimus lumborum muscle. Cryosections were stained with a Hemotoxylin-Eosin (H-E), and nicotinamide adenine dinucleotide tetrazolium reductase reaction (NADH-TR). The eventual size change of the muscle fibers were evaluated by morphometry in the H-E and NADH-TR stained cryosections, while in the NADH-TR slides the histochemical density and distribution of mitochondria were also determined. The main results of the morphometric analyses were: 1) As expected for the type of FES treatment used in this study, only a couple of horses showed significant increases in mean muscle fiber size when Pre- vs Post-FES biopsies were compared; 2) In the older horses, there were sparse (or many in one horse) very atrophic and angulated muscle fibers in both Pre- and Post-FES samples, whose attributes and distribution suggests that they were denervated due to a distal neuropathy; 3) The hypothesis of generalized FES-induced muscle fiber damage during epaxial muscle training is not supported by our data since: 3.1) Denervated muscle fibers were also present in the Pre-FES biopsies and 3.2) Only one horse presented with several long-term denervated muscles fibers Post-FES; 4) Preliminary data indicate an increased density and distribution of mitochondria in Post-FES biopsies, suggesting that the clinical improvements in the FES treated horses may be related to daily increased muscle contraction and perfusion induced by FES training. In conclusion, FES in horses is a safe treatment that provides clinical improvements in equine epaxial muscle spasms.

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Ugo Carraro, Helmut Kern, Paolo Gava, Christian Hofer, Stefan Loefler, Paolo Gargiulo, Kyle Edmunds, Íris Dröfn Árnadóttir, Sandra Zampieri, Barbara Ravara, Francesco Gava, Alessandra Nori, Valerio Gobbo, Stefano Masiero, Andrea Marcante, Alfonc Baba, Francesco Piccione, Sheila Schils, Amber Pond, Simone Mosole (2017)
Recovery from muscle weakness by exercise and FES: lessons from Masters, active or sedentary seniors and SCI patients. Aging Clinical and Experimental Research, 29(4), 579.
10.1007/s40520-016-0619-1
Seung-Min Lee, Ji-Eun Lee, Yun-Kyung Lee, Da-Ae Yoo, Da-Been Seon, Dae-Won Lee, Chang-Beom Kim, Hyuk Choi, Kwang-Ho Lee (2022)
Thermal-Corrosion-Free Electrode-Integrated Cell Chip for Promotion of Electrically Stimulated Neurite Outgrowth. BioChip Journal, 16(1), 99.
10.1007/s13206-022-00049-0
Steve Adair, Dawn Phillips (2024)
Physical Rehabilitation for Veterinary Technicians and Nurses. 429.
10.1002/9781119892441.ch23
G. Tabor, J. Williams (2020)
Objective measurement in equine physiotherapy. Comparative Exercise Physiology, 16(1), 21.
10.3920/CEP190049
Ryan Carpenter, Kimberly Johnston, Frank A. Nickels, Nancy L. Goodman, Larry R. Overly, Robin M. Dabareiner, Omar Maher, Todd C. Holbrook, Sherry A. Johnson, David D. Frisbie, David A. Wilson, Kevin G. Keegan, Jan F. Hawkins (2020)
Adams and Stashak's Lameness in Horses. 949.
10.1002/9781119276715.ch9
Roger H. Coletti (2022)
The ischemic model of chronic muscle spasm and pain. European Journal of Translational Myology, 32(1),
10.4081/ejtm.2022.10323
Diane A. Isbell, Sheila J. Schils, Suzan C. Oakley, Ugo Carraro, Brian R. Knaeble (2020)
Functional Electrical Stimulation (FES) and the Effect on Equine Multifidi Asymmetry. Journal of Equine Veterinary Science, 95, 103255.
10.1016/j.jevs.2020.103255
Daniela C. Riedler, Rebeka R. Zsoldos, Matthias Robel, Isabelle D. Jobst, Theresia F. Licka (2020)
Movement Caused by Electrical Stimulation of the Lumbosacral Region in Standing Horses. Journal of Equine Veterinary Science, 91, 103116.
10.1016/j.jevs.2020.103116
Taro Fujimaki, Takashi Ando, Takanori Hata, Yoshihiro Takayama, Tetsuro Ohba, Jiro Ichikawa, Yoshihisa Takiyama, Rikito Tatsuno, Katsuhiro Koyama, Hirotaka Haro (2021)
Exogenous parathyroid hormone attenuates ovariectomy-induced skeletal muscle weakness in vivo. Bone, 151, 116029.
10.1016/j.bone.2021.116029
Steve Adair, Dawn Phillips (2017)
Physical Rehabilitation for Veterinary Technicians and Nurses. 347.
10.1002/9781119389668.ch22
Melissa R. King, Sandro Colla (2025)
Muscle Rehabilitation Techniques and Prevention of Injury. Veterinary Clinics of North America: Equine Practice, 41(1), 193.
10.1016/j.cveq.2024.11.010
Heli K. Hyytiäinen, Anna Boström, Kjell Asplund, Anna Bergh (2022)
A Systematic Review of Complementary and Alternative Veterinary Medicine in Sport and Companion Animals: Electrotherapy. Animals, 13(1), 64.
10.3390/ani13010064
Natalia Domańska-Kruppa, Małgorzata Wierzbicka, Elżbieta Stefanik (2024)
Advances in the Clinical Diagnostics to Equine Back Pain: A Review of Imaging and Functional Modalities. Animals, 14(5), 698.
10.3390/ani14050698
Jingyi Jia, Shengbo Gong, Aili Zhang, Liping Jiang, Yifei Yao (2023)
Stiffening of the gluteal muscle increased the intramuscular stress: An in-silico implication of deep tissue injury. Heliyon, 9(2), e13459.
10.1016/j.heliyon.2023.e13459
Johanna Dietrich, Stephan Handschuh, Robert Steidl, Alexandra Böhler, Gerhard Forstenpointner, Monika Egerbacher, Christian Peham, Hanna Schöpper (2021)
Muscle Fibre Architecture of Thoracic and Lumbar Longissimus Dorsi Muscle in the Horse. Animals, 11(3), 915.
10.3390/ani11030915
Luke B Anderson, Barbara Ravara, Sohaib Hameed, Chase D Latour, Sawyer M Latour, Valerie M Graham, Mariam N Hashmi, Brittan Cobb, Nicole Dethrow, Albert K Urazaev, Judy K Davie, Giovanna Albertin, Ugo Carraro, Sandra Zampieri, Amber L Pond (2021)
MERG1A Protein Abundance Increases in the Atrophied Skeletal Muscle of Denervated Mice, But Does Not Affect NFκB Activity. Journal of Neuropathology & Experimental Neurology, 80(8), 776.
10.1093/jnen/nlab062
Steve Adair, Tena Ursini (2024)
Equine Sports Medicine and Surgery. 609.
10.1016/B978-0-7020-8370-9.00029-1
Sheila Schils, Ugo Carraro, Tracy Turner, Barbara Ravara, Valerio Gobbo, Helmut Kern, Lin Gelbmann, Jamie Pribyl (2015)
Functional Electrical Stimulation for Equine Muscle Hypertonicity: Histological Changes in Mitochondrial Density and Distribution. Journal of Equine Veterinary Science, 35(11-12), 907.
10.1016/j.jevs.2015.08.013
Melinda R. Story, Kevin K. Haussler, Yvette S. Nout-Lomas, Tawfik A. Aboellail, Christopher E. Kawcak, Myra F. Barrett, David D. Frisbie, C. Wayne McIlwraith (2021)
Equine Cervical Pain and Dysfunction: Pathology, Diagnosis and Treatment. Animals, 11(2), 422.
10.3390/ani11020422
Sheila Schils, Timothy Ober (2022)
Functional Electrical Stimulation (FES) in the Diagnosis and Treatment of Musculoskeletal and Neuromuscular Control Abnormalities in Horses - Selected Case Studies. Journal of Equine Veterinary Science, 117, 104078.
10.1016/j.jevs.2022.104078

Supporting Agencies

Brian Knaeble, Statistics, University of Wisconsin-Stout
Sheila Schils, Equine Rehabilitation LLC, River Falls, Wisconsin
former Professor University of Wisconsin-River Falls

How to Cite

Ravara, B., Gobbo, V., Carraro, U., Gelbmann, L., Pribyl, J., & Schils, S. (2015). Functional electrical stimulation as a safe and effective treatment for equine epaxial muscle spasms: Clinical evaluations and histochemical morphometry of mitochondria in muscle biopsies. European Journal of Translational Myology, 25(2), 109–120. https://doi.org/10.4081/ejtm.2015.4910