The role of an herbal agent in treatment for Escherichia coli induced bacterial cyctitis in rats


Submitted: February 3, 2017
Accepted: April 3, 2017
Published: June 30, 2017
Abstract Views: 1693
PDF: 1402
Publisher's note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Authors

  • Murat Tuken Bagcilar Training & Research Hospital, Dept. of Urology, Istanbul, Turkey.
  • Mustafa Zafer Temiz Bagcilar Training & Research Hospital, Dept. of Urology, Istanbul, Turkey.
  • Emrah Yuruk Bagcilar Training & Research Hospital, Dept. of Urology, Istanbul, Turkey.
  • Asuman Orcun Kaptanagasi Kartal Lütfi Kirdar Training & Research Hospital, Dept. of Biochemistry, Istanbul, Turkey.
  • Kayhan Basak Kartal Lütfi Kirdar Training & Research Hospital, Dept. of Pathology, Istanbul, Turkey.
  • Fehmi Narter Kartal Lütfi Kirdar Training & Research Hospital, Dept. of Urology, Istanbul, Turkey.
  • Ahmet Yaser Muslumanoglu Bagcilar Training & Research Hospital, Dept. of Urology, Istanbul, Turkey.
  • Kemal Sarica Kartal Lütfi Kirdar Training & Research Hospital, Dept. of Urology, Istanbul, Turkey.
Objectives: The aim of this study is to evaluate the effects of the herbal agent in the prevention and treatment of bacterial cystitis in a rat model. Material and Methods: A total of twenty-eight male Sprague- Dawley rats were divided into four groups. Group-1 constituted the control group (operated and normal saline injected into the bladder, received only drinking water for 7 days); Group-2 constituted the no-treatment group (operated, E.coli J96 strain injected into the bladder, received only drinking water for 7 days); Group-3 constituted the short-term treatment (operated, E.coli J96 strain injected into the bladder, received the herbal agent added into drinking water for 7 days) and Group-4 constituted the long-term treatment (operated, E. coli J96 strain injected into the bladder, received herbal agent added into drinking water for 14 days). At the end of the pre-defined treatment periods of duration, the rats were sacrificed, urine samples collected from the bladder for culture and bladders were harvested for histopathological evaluation. Urine culture results and histopathological findings were comparatively evaluated between the groups. Results: Urine cultures were positive for implanted E. coli strains in 0%, 85.7%, 42.8% and 0% of rats in Group 1, Group 2, Group 3 and Group 4, respectively (p = 0.001). Although histopathological evaluation revealed increased vascular dilation in the bladder specimens obtained from Group 2 and Group 3 (p = 0.028) no significant difference was noticed in level of inflammation (p = 0.610), edema (p = 0.754) and thickness of uroepithelium (p = 0.138). Conclusion: While long term (14 days) treatment with an herbal agent added into the drinking water resulted in complete clearance of urine from E. coli; shorter application of the agent revealed partial clearance. Further clinical studies are needed to support our results.

Tuken, M., Temiz, M. Z., Yuruk, E., Kaptanagasi, A. O., Basak, K., Narter, F., Muslumanoglu, A. Y., & Sarica, K. (2017). The role of an herbal agent in treatment for Escherichia coli induced bacterial cyctitis in rats. Archivio Italiano Di Urologia E Andrologia, 89(2), 134–138. https://doi.org/10.4081/aiua.2017.2.134

Downloads

Download data is not yet available.

Citations