Cover Image

Exploring the world of micro sculptures - subfossil Cladocera remains under the SEM

Izabela Zawiska, Edyta Zawisza, Marta Wojewódka, Artem Y. Sinev
  • Izabela Zawiska
    Polish Academy of Sciences, Department of Geoecology and Climatology, Poland | izawiska@twarda.pan.pl
  • Edyta Zawisza
    Polish Academy of Sciences, Institute of Geological Sciences, Poland
  • Marta Wojewódka
    Polish Academy of Sciences, Institute of Geological Sciences, Poland
  • Artem Y. Sinev
    Lomonosov Moscow State University, Department of Invertebrate Zoology, Russian Federation

Abstract

The scanning electron microscope (SEM) is widely used for the identification of microstructural characteristics and morphology of different microorganisms. Common procedures are based and developed for remains of living species. This paper presents an effective method for drying and preparing subfossil Cladocera remains for SEM observation, which has been recently adapted and tested on several samples originating from different American and European lakes. This method results to be fast and cheap, as it excludes the use of expensive and toxic reagents. Moreover, it allows to recognize the micro sculpture and other species specific characteristics present on the different body parts of the Cladocera remains. The present contribution provides 29 high quality pictures of 12 cladoceran species at magnification between 200x and 11,000x. SEM images reveal  that the patterns observed on the shells under the light microscope actually are always three dimensional structures.

Keywords

SEM; subfossil Cladocera; micro sculpture; chitin.

Full Text:

PDF
HTML
Submitted: 2016-08-03 14:44:49
Published: 2016-12-29 09:08:09
Search for citations in Google Scholar
Related articles: Google Scholar
Abstract views:
416

Views:
PDF
187
HTML
477

Article Metrics

Metrics Loading ...

Metrics powered by PLOS ALM


Copyright (c) 2016 Izabela Zawiska, Edyta Zawisza, Marta Wojewódka, Artem Y. Sinev

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
 
© PAGEPress 2008-2018     -     PAGEPress is a registered trademark property of PAGEPress srl, Italy.     -     VAT: IT02125780185